C5990 Applied enzymology

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Timetable
Thu 13:00–14:50 C05/107
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.
Syllabus
  • 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Assessment methods
lectures, oral examination, alternatively colloquium
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Autumn 2008, recent)
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