C5990 Applied enzymology

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
Learning outcomes
Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
Syllabus
  • 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
Teaching methods
lecture course, finished by examination
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Spring 2008 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Spring 2006, Autumn 2006, Spring 2007, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (recent)
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