C8111 Bioengineering

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Martin Mandl, CSc. (lecturer)
Guaranteed by
doc. Ing. Martin Mandl, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Tue 12:00–13:50 online_BCH2
Prerequisites
Bachelor degree in biochemistry or related fields
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquire basic bioengineering knowledge enabling to understand and control the processes of biochemical engineering for the needs of biotechnological research and development and for application in industrial practice.
Learning outcomes
Student will be able to: - be knowledgeable about the main findings in biochemical engineering under laboratory and industrial scale; - understand basic processes and equipment in biotechnology using microbial and other cell cultures and enzymes; - control the basics of bioprocess kinetics to describe and model its course in bioreactors.
Syllabus
  • Bioengineering - biochemical engineering in controlling biotechnological processes. Biocatalysts in biotechnology, from enzymes to microbial, plant and animal cells, relationships between enzyme and cell kinetics. • Bioreactors as universal cultivation and production devices. Main features and equipment of bioreactors. • Scale up. Development of biotechnology process from laboratory to industrial scale. • Specificity of bioreactors and culture devices for plant and animal cultures. • Processes in bioreactors. Stirring and aeration, determination of optimal parameters in terms of economics and bio-process biochemistry. • Critical aeration parameters in terms of substrate oxidation and overall bioprocess kinetics. • Sterilization of materials, nutrient media and air. • Biomass production. Batch and fed-batch cultures, bioprocess constants. • Kinetic models of biomass growth, substrate consumption and product formation. Cell death and autolysis. Application of a suitable model. • Continuous cultivation. Steady state, determination of the kinetic parameters under specific conditions of continuous process. • Use of the batch process conditions to determine the optimal parameters of continuous culture. • Bioreactors with immobilized biocatalysts. Application of enzymes and cells, kinetics of product formation. • Bioprocess monitoring, separation of biomass and products.
Literature
  • KAŠTÁNEK, František. Bioinženýrství. Vyd. 1. Praha: Academia, 2001, 334 s. ISBN 8020007687. info
  • POSTEN, Clemens. Integrated bioprocess engineering. Berlin: De Gruyter, 2018, xi, 344. ISBN 9783110315387. info
  • SHULER, Michael L., Fikret KARGI and Matthew DELISA. Bioprocess engineering : basic concepts. Third edition. Boston: Prentice-Hall, 2017, xxi, 632. ISBN 9780137062706. info
  • STANBURY, Peter F., Allan WHITAKER and Stephen J. HALL. Principles of fermentation technology. 2nd ed. Oxford: Pergamon, 1995, xviii, 357. ISBN 0080361323. info
  • DORAN, Pauline M. Bioprocess engineering principles. Second edition. Amsterdam: Academic Press, 2013, viii, 919. ISBN 9780122208515. info
  • KATOH, Shigeo, Jun-ichi HORIUCHI and Fumitake YOSHIDA. Biochemical engineering : a textbook for engineers, chemists and biologists. Second, completely revised a. Weinheim: Wiley-Vch, 2015, xxii, 303. ISBN 9783527338047. info
  • MCDUFFIE, Norton G. Bioreactor design fundamentals. Boston: Butterworth-Heinemann, 1991, ix, 137. ISBN 0750691077. info
  • BAILEY, James E. and David F. OLLIS. Biochemical engineering fundamentals. 2nd edition. Tamil Nadu: McGraw-Hill, 1986, xxi, 984. ISBN 0070701237. info
  • Basic biotechnology. Edited by Colin Ratledge - Bjørn Kristiansen. 3rd ed. Cambridge: Cambridge University Press, 2006, xiv, 666. ISBN 9780521549585. URL info
  • Biology and engineering of stem cell niches. Edited by Ajaykumar Vishwakarma - Jeffrey Karp. London: Academic Press, 2017, xv, 625. ISBN 9780128027349. info
  • Stem cell and tissue engineering. Edited by Song Li - Nicolas L'Heureux - Jennifer H. Elisseeff. New Jersey: World Scientific, 2011, xxiv, 448. ISBN 9789814317054. info
Teaching methods
Lectures on selected parts of bioengineering. Discussions on detailed subjects.
Assessment methods
Oral exam.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2020, Spring 2022, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Spring 2021, recent)
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