Bi6126 Laboratory sampling for aDNA analysis

Faculty of Science
Spring 2022
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Eva Chocholová (seminar tutor)
Mgr. Dana Buriánková Fialová, Ph.D. (seminar tutor)
Mgr. et Mgr. Kristýna Brzobohatá, Ph.D. (seminar tutor)
doc. RNDr. Eva Drozdová, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Eva Drozdová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Eva Chocholová
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology && Bi6400c Molecular Methods - practice
The course is designed for students of master's degree of Antropobiologie and antropogenetika. Student will learn during the laboratory exercise all steps of aDNA analysis.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
The student gains practical experience with sterile sampling of DNA from historical skeletal material. He will be trained in the work of a DNA sample in a laboratory. This exercise is necessary for students of anthropobiology and anthropogenetics to develop their diploma theses.
Learning outcomes
Students completing the course will be able to: - separate tissue sampling for DNA analysis - lysing bone tissue, purifying and DNA - choose the most suitable method for the given topic of the diploma thesis - design a protocol for genetic analysis - optimize a protocol for genetic analysis - bioinformatically and statistically evaluate the results
Syllabus
  • 1. Selection of sample for analysis of DNA, anti-contaminating measures 2. Lysis, purification of a DNA 3. Quantification and determination of aDNA quality 4. PCR and its optimization 5. Sequencing 6. Bioinformatics and statistical tools for evaluation of genetic analysis results
Literature
  • terénní cvičení bez doporučené literatury
  • Ancient DNA : recovery and analysis of genetic material from paleontological, archaeological, museum, medical, and forensic specimens. Edited by Bernd Herrmann - Susanne Hummel. New York: Springer-Verlag, 1994, xi, 263. ISBN 0387943080. info
Teaching methods
laboratory practise
Assessment methods
active participation
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/Bi6126