Bi3010 Electron microscopy

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Iveta Hodová, Ph.D. (lecturer)
Guaranteed by
Mgr. Iveta Hodová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Iveta Hodová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science
Timetable
Mon 12:00–13:50 B11/306
  • Timetable of Seminar Groups:
Bi3010/01: Tue 9:00–10:50 D31/1S11, I. Hodová
Bi3010/02: Tue 11:00–12:50 D31/1S11, I. Hodová
Bi3010/03: Tue 14:00–15:50 D31/1S11, I. Hodová
Bi3010/04: Wed 9:00–10:50 D31/1S11, I. Hodová
Bi3010/05: Wed 11:00–12:50 D31/1S11, I. Hodová
Bi3010/06: Wed 14:00–15:50 D31/1S11, I. Hodová
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 70 student(s).
Current registration and enrolment status: enrolled: 12/70, only registered: 0/70, only registered with preference (fields directly associated with the programme): 0/70
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The main purpose of the course is to provide students with a general overview of the basic techniques of electron microscopy and of the possibilities of processing biological material for electron microscopes.
Learning outcomes
At the end of this course, students should be able to explain the principles and application of the electron microscopy, the history of the development of electron microscopes; to describe the construction of transmission electron microscope (TEM) and scanning electron microscope (SEM); to understand and explain the imaging methods in TEM and SEM; to learn basic techniques of specimen preparation for electron microscopy; to apply the knowledge to the selected samples; to interpret biological structures in the electron micrographs; to have knowledge of artefacts; to acquire the principles of the routine operation of the electron microscope.
Syllabus
  • The theoretical part:
  • an introduction to electron microscopy
  • transmission electron microscope
  • preparative methods for TEM
  • scanning electron microscope
  • preparative methods for SEM
  • electron microscopic cytology
  • The practical part:
  • preparing glass knives
  • preparing grids for ultrathin sections
  • cutting and staining of semi-thin sections
  • preparing samples for SEM
  • individual routine operation of the electron microscope
Literature
  • KALINA, Tomáš and Vladimír POKORNÝ. Základy elektronové mikroskopie : pro biology. 1. vyd. Praha: Univerzita Karlova, 1979, 206 s. info
  • MRÁZ, Peter and Jozef POLÓNYI. Metódy elektronovej mikroskopie živočíšnych tkanív. Bratislava, 1988. info
Teaching methods
theoretical lectures, laboratory practice, individual routine operation of the electron microscope
Assessment methods
Colloquium consists of oral exam, evaluation of protocols, determination and assessment of 5 electron micrographs
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Information on course enrolment limitations: 6 skupin po 10 studentech
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Autumn 2002, Autumn 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/Bi3010