Bi6150c Mycorrhizal symbioses - practice

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Milan Baláž, Ph.D. (seminar tutor)
Guaranteed by
RNDr. Milan Baláž, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 15:00–16:50 C13/332
Prerequisites (in Czech)
NOW( Bi6150 Mycorrhizal symbioses )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to famaliarize students with basic methods of mycorrhizal symbioses research.
Learning outcomes
At the end of this practical course, students are able to:
distinguish mycorrhizal symbioses on the base of their morphology and anatomy;
evaluate the effects of mycorrhizal symbiosis on plant growth;
quantify the extent of root colonization and external mycelia growth;
cultivate and propagate the mycorrhizal fungi.
Syllabus
  • Mycorrhizal colonization of plant roots: commonly used methods, assessment of root colonization (chemical and microscopic methods), observation of typical mycorrhizal structures.

  • Isolation of spores of arbuscular and orchid fungi: isolation techniques, staining by Melzer's reagent, sample preparation, spore description and fungal species determination.

  • Mycorrhizal extraradical mycelium: extraction techniques (membrane filtration and inserted membrane technique), vital staining techniques, assessment of extraradical hyphal length (grid-line intersect method and image analysis).

  • Long-term cultivation experiment: the aim of the experiment vary with every course run.

Literature
  • GRYNDLER, Milan, Milan BALÁŽ, Hana HRŠELOVÁ, Jan JANSA and Miroslav VOSÁTKA. Mykorhizní symbióza. O soužití hub s kořeny rostlin (Mycorrhizal symbiosis. On the coexistence of fungi with plant roots.). 1. vydání. Praha: Academia, 2004, 366 pp. 1. vydání. ISBN 80-200-1240-0. info
  • NORRIS, JR, DJ READ and AK VARMA. Techniques for the study of mycorrhiza, Part 1. London: Academic Press, 1991. info
Teaching methods
Practical laboratory course.
Assessment methods
Active participation at the course, elaboration of protocols.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Teacher's information
http://elanor.sci.muni.cz/~mykorrhi
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/Bi6150c