Bi8670c Plant Biotechnology Principles - Practical Course

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Markéta Šámalová, Ph.D. (seminar tutor)
Mgr. Hana Cempírková, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Markéta Šámalová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Markéta Šámalová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 13:00–14:50 C13/112
Prerequisites (in Czech)
NOW( Bi8670 Plant Biotechnology ) && SOUHLAS
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The subject follows on from the course Bi6120 Plant explants, in which students were introduced to individual methods of in vitro cultivation. It expands knowledge towards the use of these methods in basic plant research and biotechnological applications. The subject will focus on the basic methods of molecular biology used for the preparation of genetically modified organisms (GMOs), including transgenic plants, and targeted modification of plant genome (e.g. CRISPR/Cas9).
Learning outcomes
After completing this course, the student will acquire theoretical and practical knowledge and be able to: • understand and describe the main methods used in molecular biology for the preparation of GMOs • master the preparation of transgenic plants and their analysis, including fluorescence and confocal microscopy • understand and analyze benefits and risks of modern plant biotechnologies.
Syllabus
  • • Basic procedure for the preparation of genetically modified plants • Genomic databases, primer design, PCR • Isolation of plasmid DNA, its cleavage and electrophoresis • Basics of cloning • Techniques for the transformation of E. coli and agrobacteria • Transformation of tobacco by the in vitro method • Transformation of Arabidopsis thaliana by the floral dip method • Chemically inducible systems, GUS staining • Selection of transformants • Fluorescent proteins and fluorescence microscopy • Method of targeted genome modification (CRISPR/Cas9) • Transient expression in tobacco and confocal microscopy • Benefits and risks of GMOs, legislation.
Literature
  • Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Zhang, X., Henriques, R., Lin, S.-S., Niu, Q.-W. and Chua, N.-H. NATURE PROTOCOLS 2006, 1:1-6.
  • Editing plant genomes with CRISPR/Cas9. Belhaj, K., Chaparro-Garcia, A., Kamoun, S., Patron, N.J. and Nekrasov, V. Current Opinion in Biotechnology 2015, 32:76–84.
  • ŠÁMALOVÁ, Markéta, C. KIRCHHELLE and I. MOORE. Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species. 2019. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20089. URL info
  • Applied plant cell biology : cellular tools and approaches for plant biotechnology. Edited by Peter Nick - Zdeněk Opatrný. Berlin: Springer, 2014, xiv, 481. ISBN 9783642417863. info
  • ALBERTS, Bruce, Alexander JOHNSON, Julian LEWIS, David Owen MORGAN, Martin C. RAFF, Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Sixth edition. New York, NY: Garland Science, 2014, xxxiv, 134. ISBN 9780815344322. info
  • Medicinal plant biotechnology. Edited by Rajesh Arora. Cambridge, Mass.: CABI, 2010, xv, 357 p. ISBN 9781845936921. info
  • SAMBROOK, Joseph and David W. RUSSELL. Molecular cloning : a laboratory manual. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2001, 1 sv. ISBN 0879695765. info
Teaching methods
praktická výuka
Assessment methods
Test.
Language of instruction
Czech
Further comments (probably available only in Czech)
General note: Předmět je vypisován v jarních semestrech sudých let.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2016, spring 2018, Spring 2019.
  • Enrolment Statistics (recent)
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