PřF:C7876en Gen.tech.lab - Course Information
C7876en Gene technologies - lab.course
Faculty of ScienceSpring 2025
- Extent and Intensity
- 0/0/5. 5 credit(s). Type of Completion: z (credit).
In-person direct teaching - Teacher(s)
- doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
- Guaranteed by
- doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- C7875 Gen.tech
Passing of course C7875 Gene technologies. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biotechnology (programme PřF, N-BTC)
- Course objectives
- The laboratory course aims to provide students with practical skills in methods of recombinant DNA technologies, genetic manipulation with bacteria and yeasts, expression of recombinant proteins in E. coli and P. pastoris, bacterial genome sequencing, and transcriptomic and metagenomics analysis.
- Learning outcomes
- The student will have the following practical skills: 1. Design and implement techniques related to DNA manipulation (cloning, transformation) 2. Design and perform expression of recombinant proteins in the E. coli and P. pastoris yeast expression system 3. To perform and evaluate metagenomics analysis from the environmental sample. 4. To perform and do a basic evaluation of bacterial genome and transcriptome sequencing data. 5. To perform a critical evaluation of acquired data within basic methods of current gene technologies
- Syllabus
- Exercise 1 - Expression of Aspartate-tRNA Ligase Protein in E. coli BL21 (DE3) and its purification by affinity chromatography (IMAC) Exercise 2 - Expression of protein elicitin in P. pastoris GS115 and its subsequent purification by ion-exchange chromatography Exercise 3 - Metagenomics analysis of the environmental sample on the MiniSeq NGS instrument Exercise 4 - Analysis of bacterial transcriptome changes on the MiniSeq NGS instrument Exercise 5 - Bacterial genome sequencing on the MiniSeq NGS instrument Exercise 6 - Evaluation of acquired sequencing data.
- Teaching methods
- Practical course
- Assessment methods
- After completing all exercises, an oral presentation presenting measured data and individual problems is done.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2025/C7876en