C8113 Biotechnology methods

Faculty of Science
Spring 2025
Extent and Intensity
0/0/5. 5 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Jiří Kučera, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C7875 Gen.tech &&NOW( C7876 Gen.tech.lab )
Lab course Methods in Biotechnology (C8113) is limited in capacity and is intended only for first-year students of the Master's program in Biotechnology (N-BTC Faculty of Science). The teaching format is based on project-based learning in collaboration with the Gene Technology lab course (C7876). Both courses must be co-registered in the spring semester.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aim of the course is to acquire basic methods used in biotechnology. In practical exercises, students will be familiarized with the procedures typical for developing and realizing the biotechnology process in a bioreactor with subsequent purification of the product.
Learning outcomes
Students will know the basic principles of applying selected methods used in biotechnology and use different methods for further experimental work in biotechnology and related fields.
Syllabus
  • Preparation of laboratory benchtop and semi-operational bioreactor for culturing of microorganisms. Batch and fed-batch cultivation of microorganisms. Monitoring of important bioprocess parameters such as DO, OD, pH, and temperature. Production of recombinant proteins using microbial expression systems and subsequent purification of target proteins by FPLC. Determination of metabolites using advanced HPLC-Q-TOF, MIMS, and immunochemical techniques.
Literature
    recommended literature
  • POSTEN, Clemens. Integrated bioprocess engineering. Berlin: De Gruyter, 2018, xi, 344. ISBN 9783110315387. info
  • Protein purification : principles, high resolution methods, and applications. Edited by Jan-Christer Janson. 3rd ed. Hoboken, N.J.: John Wiley & Sons, 2011, xiv, 517. ISBN 9780471746614. info
  • Methods in biotechnology. Edited by Hans-Peter Schmauder. London: Taylor & Francis, 1997, ix, 257 s. ISBN 0-7484-0429-5. info
Teaching methods
Laboratory course guided by project-based learning.
Assessment methods
Compulsory participation during the semi-block course. Information about the class schedule will be communicated at the information meeting at the beginning of the semester. Compulsory participation in a one-day excursion at a biotechnology company organized by the school. Compulsory participation in the final seminar, where students comment on their results in the form of a PowerPoint talk.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: in blocks.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2025/C8113