LF:MBKI081p Klinická imunologie II - př. - Course Information

MBKI081p Klinická imunologie II - přednáška

Extent and Intensity

2/0. 4 credit(s). Type of Completion: zk (examination).

Teacher(s)

doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Mgr. Marcela Vlková, Ph.D. (lecturer)
Michaela Gregorovičová (assistant)

Guaranteed by

doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Prerequisites

Bachelor's studies focused on laboratory medicine or natural sciences

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Bioanalyst - Specialist in Laboratory Methods (programme PřF, N-BCH)

Course objectives

The aim is to provide basic information on the structural and functional characteristics of human immune system, physiology and immunity pathology, immunological treatment and prevention, possibilities and methods of laboratory assessment of immune status in health and illness.

Learning outcomes

Upon completion of this course the student will understand basic mechanisms of immune response so that he can understand how development of immune system disorders leads to various forms of immunopathological diseases as well as possibilities of laboratory immunological diagnostics. Student will be able to make decisions about the suitability of immunological tests in specific situations.

Syllabus

• Theoretical part - basics of immunology Immune system: nomenclature, general characteristics. Cells, tissues and organs of the immune system. Congenital immunity. Recognition of extraneous biomacromolecules. Ingredients of innate immunity (epithelial barriers, professional phagocytes, NK cells, complement system, cytokines, acute phase proteins). Inflammatory reactions as the basic defense response of the organism. Basic features of adaptive immune response. Antigen. Immunological specificity. Immunological memory. Immunological tolerance. The course of the immune reaction. Structure and function of the major histocompatibility complex (MHC - HLA). Cells presenting antigen. Presentation of antigen to immunologically competent cells. Central and peripheral lymphatic organs. Development of lymphocytes T and B. Lymphocyte receptors T and B for antigen. Emergence of immune repertoire. T cell lymphocyte mediated immune responses T. Lymphocyte Polarization T. Effect and regulatory functions of lymphocytes T. Humoral immune reaction. Activation of T lymphocytes and antibody production. Immunoglobulins - structure and function. Mucosal immune system. Regionalization of immune reactions. Effector mechanisms of immune responses leading to protection and damage to the body. Anti-infective immunity. The importance of microorganisms for the immune system. Immune system in cancer diseases. Tumor antigens. Tumors from the immune system cells. Transplantation Immunology. Immunological aspects of organ transplantation and bone marrow. Blood transfusion. Immunology of human reproduction. Physiological and pathological autoimmune reactions. Autoimmune diseases. Allergic diseases. Allergens. Atopic diathese. Primary and secondary immunodeficiency. AIDS. Immunotherapy and immunomodulation. Treatment with immunoglobulin preparations. Immunosuppression. Gene therapy of immunological diseases. Vaccines and vaccination. Immune system - nervous system - endocrine system - external environment. Theoretical part - immunological investigation methods Antigen preparations. Immunization. Getting serum Isolation of immunoglobulins. Monoclonal antibodies. In vitro antigen-antibody response, visualization capabilities. Radial immunodiffusion. Nephelometry. Immunoelectrophoresis, immunofixation, western blot. Immunofluorescence. Chemiluminiscence. Immuno-enzymatic reactions. ELISA. Radioimmunoassays. Immunohistological techniques. Isolation of immune system cells from blood and other biological materials. Cryopreservation of cells. Flow cytometry. Immunophenotyping of lymphocytes and other cells of the immune system. Separation of cell subpopulations (immunomagnetic and other methods) Cell cultures in immunology. Proliferation of lymphocytes and detection options. Detection of lymphocyte cytotoxicity. In vitro antibody production. ELISPOT. Phagocytosis tests (chemotaxis, respiratory flare, microbicides). Molecular-genetic techniques used in immunology (Southern-, Northern-blotting, PCR). Principles of proper laboratory work. Internal and external quality control system.

Literature

• LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2011, 53 pp. ISBN 978-80-210-4227-8. info
• BARTŮŇKOVÁ, Jiřina and Milan PAULÍK. Vyšetřovací metody v imunologii. Vyd. 1. Praha: Grada, 2005, 176 s. ISBN 8024706911. info
• HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 3. vyd. Praha: Triton, 2005, 279 s. ISBN 8072546864. info
• ABBAS, Abul K. and Andrew H. LICHTMAN. Basic immunology : functions and disorders of the immune system. Illustrated by David L. Baker - Alexandra Baker. 2nd ed. Philadelphia: Saunders, 2004, ix, 322. ISBN 072160241X. info

Teaching methods

lectures with the use of audiovisual aids (presentation, commented videos), including interactive inputs

Assessment methods

oral examination

Language of instruction

Czech

Further Comments

Study Materials

• Enrolment Statistics (Spring 2017, recent)
  
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