aVLMB031 Modern Imaging and Analytical Methods in Cellular Biology and Immunology

Faculty of Medicine
autumn 2020
Extent and Intensity
0/0.7/.3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Andrejčinová, Ph.D. (seminar tutor)
Mgr. Jan Frič, Ph.D., MHA (seminar tutor)
Sebastiano Giallongo, Ph.D. (seminar tutor)
Mgr. Petra Lázničková, Ph.D. (seminar tutor)
Sofia Morazzo (seminar tutor)
Gretsen Velezmoro Jauregui, M.Sc., Ph.D. (seminar tutor)
Mgr. Ondřej Vymazal, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Jan Frič, Ph.D., MHA
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Mgr. Jan Frič, Ph.D., MHA
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
aVLMB031/A: Thu 5. 11. 9:00–16:00 A 1.08, Fri 6. 11. 9:00–13:00 A 1.08
aVLMB031/B: Thu 12. 11. 9:00–16:00 A 1.08, Fri 13. 11. 9:00–13:00 A 1.08
Prerequisites
VSBI0222p Biology II-lect. || aVLBI0222p Medical Biology II - lect. || ZLBI0222p Biology II -lect. || aZLBI0222p Biology II - lecture
Students need to have cell biology knowledge at the level of 1st year of university, proven with an exam (aVLBI0222p, aZLBI0222p or Bi1700).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 40 student(s).
Current registration and enrolment status: enrolled: 3/40, only registered: 0/40
fields of study / plans the course is directly associated with
Course objectives
After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); confocal microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.
Learning outcomes
After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); confocal microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.
Syllabus
  • Principles of confocal microscopy and applications
  • Principles of flow cytometry and cell phenotype analysis using flow cytometry
  • Cell separation methods (FACS sorting)
  • Advanced flow cytometry methods, Spectral cytometry, Mass cytometry
  • Advanced microscopy techniques - electron and multiphoton microscopy
  • Other molecular biology methods for proteins and RNA analysis
Literature
  • Relevant textbooks chapters and links to internet sources that will help to understand the basic principles of the methods will be provided during the course.
Teaching methods
a seminar, lab demonstration
Assessment methods
Course completion requires full atendance in the seminars and passing a final written test (10 questions about basic principle of demonstrated methods).
Attendance at all seminars is mandatory.
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 15.
Teacher's information
https://is.muni.cz/auth/el/1411/podzim2016/VLMB011/index.qwarp
The course is also listed under the following terms Autumn 2017, autumn 2018, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2020/aVLMB031