LF:VLBC0321p Biochemistry I - lecture - Course Information

VLBC0321p Biochemistry I - lecture

Extent and Intensity

3/0/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Jiří Dostál, CSc. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
doc. Mgr. Zdenka Fohlerová, Ph.D. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
PharmDr. Kristýna Šebrlová, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)

Guaranteed by

doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine

Timetable

Mon 30. 10. to Fri 22. 12. Tue 10:00–11:50 B22/116 aula, Mon 18. 9. to Fri 20. 10. Tue 10:00–11:50 B22/116 aula, Thu 9:00–9:50 B22/116 aula; and Tue 24. 10. 10:00–11:50 Kinosál N02901

Prerequisites (in Czech)

VLBI0222p Biology II-lect. && VLBF011p Biophysics-lect.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• General Medicine (programme LF, M-VL) (2)

Course objectives

The aim of the subject Biochemistry I is to gain knowledge about the basic metabolic processes and pathways taking place at the cellular level.
Understanding these processes is the basis for understanding metabolism at the tissue and organ level, which is studied in the follow-up subject Biochemistry II.

Learning outcomes

At the end of the course, students will:
- understand the meaning of basic chemical concepts and can apply this knowledge to describe biochemical processes in the body and the properties of body fluids.
- discuss the properties and function of enzymes.
- describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level, and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discuss the principles of some diseases at the molecular level.

Syllabus

• Electrolytes, ion activity. Weak acids/bases. Buffers. Bioenergetics, Gibbs energy, High-energy compounds. Redox reaction.
• Enzymes. Characteristic features of biocatalysis. Cofactors.
• Metabolism of nutrients. Citrate cycle. Respiratory chain. Oxidative phosphorylation.
• Carbohydrates. Carbohydrates in nutrition. Carbohydrate digestion. Basic features of glucose metabolism. Glucose transport into cells. Glycolysis. Gluconeogenesis.
• Glycogen. Metabolism of fructose, galactose, lactose. The pentose phosphate pathway. Glycosaminoglycans, proteoglycans.
• Lipids and fatty acids. Surfactants. Lipophilic vitamins. Lipids in nutrition. Lipid digestion. Fatty acid metabolism. PUFA synthesis. Metabolism of ketone bodies.
• Metabolism of triacylglycerols, glycerophospholipids, sphingolipids. Lipoperoxidation. Biosynthesis and importance of eicosanoids. Metabolism of cholesterol, bile acids. Biosynthesis of calciols.
• Amino acids. Peptides. Structure of proteins, myosin, collagen, immunoglobulins. Hemoglobin, myoglobin. Hemoglobinopathies.
• Digestion and intracellular degradation of proteins. General features in catabolism of amino acids. Ammonia metabolism and toxicity.
• Catabolism of amino acids, congenital disorders of amino acid metabolism. Synthesis of non-essential amino acids.
• Conversions of amino acids into low molecular weight products. Heme synthesis, porphyria.
• Metabolism of purine/pyrimidine nucleotides.
• Structure of DNA, RNA. Replication and corrections. Transcription and its regulation, post-transcriptional modifications.
• Protein synthesis. Folding, transport and sorting of proteins in the cell. Post-translational modification.
• Biosynthesis of collagen, elastin.

Literature

required literature
• MURRAY, Robert K., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY, Victor W. RODWELL and P. Anthony WEIL. Harperova ilustrovaná biochemie. Translated by Bohuslav Matouš. Páté české vydání, prv. Praha: Galén, 2012, xii, 730. ISBN 9788072629077. info

recommended literature
• LIEBERMAN, Michael and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 5th edition. Philadelphia: Wolters Kluwer, 2018, xii, 1051. ISBN 9781496387721. info
• KOOLMAN, Jan and Klaus-Heinrich RÖHM. Barevný atlas biochemie. Translated by Vladimír Benda - Martin Vejražka - Jiří Jonák. 1. české vydání. Praha: Grada, 2012, xiv, 498. ISBN 9788024729770. info
• VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Úvod do všeobecnej a klinicky aplikovanej biochémie. Edited by Jozef Čársky. Prvé slovenské vydanie. Bratislava: Balneotherma s.r.o., 2015, 669 stran. ISBN 9788097015688. info

Teaching methods

Lectures.

Assessment methods

The student passes to the follow-up course Biochemistry II in the following semester without the obligation to test the knowledge.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 45.

• Enrolment Statistics (autumn 2023, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2023/VLBC0321p