BLMB011c Molecular and Cellular Biology - practice

Faculty of Medicine
autumn 2024
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Kateřina Cetkovská, Ph.D. (lecturer)
Ing. Lívia Eiselleová, Ph.D. (lecturer)
Mgr. Kateřina Francová, Ph.D. (lecturer)
prof. RNDr. Ondřej Slabý, Ph.D. (lecturer)
Mgr. Kateřina Straková, Ph.D. (lecturer)
doc. Mgr. Jiří Šána, Ph.D. (lecturer)
Jana Růžičková (assistant)
Guaranteed by
doc. Mgr. Jiří Šána, Ph.D.
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Mgr. Kateřina Francová, Ph.D.
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine
Timetable
Wed 14:30–15:30 B07/205a, Wed 15:30–16:30 B07/217, Wed 15:30–16:30 B07/205a, Wed 16:30–17:00 B07/205a
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are:
- learning to operate the light microscope while observing prokaryotic and eukaryotic cells and various cell structures and processes
- understanding the methods of risk evaluation for monogenic hereditary diseases in patients based on family anamneses
Learning outcomes
After completing the course the student will be able to:
- operate the light microscope
- perform some of the basic cytochemical staining techniques in observation of bacterial and human cells
- explain the basic principles and use of electron microscopy
- describe the basic techniques and applications of tissue and cell cultures
- deduce the impact of selected DNA mutations on the primary structure of proteins
- explain the methodology of assembling and examining the karyotype and its use, and to evaluate basic numerical and structural aberrations in assigned human karyotypes
- evaluate the risk of numerical and structural aberrations resulting from meiotic non-disjunctions and chromosomal translocations
- evaluate the risk of monogenic hereditary diseases with autosomal inheritance in patients based on assigned family anamneses of the diseases
Syllabus
  • Instructions. Non-cellular organisms and infectious agents
  • Prokaryotic cells and their medical significance, basics of light microscopy
  • Microscopic observation of eukaryotic cells
  • In vitro culture of human cells
  • Principles of electron microscopy, cellular ultrastructure
  • DNA structure, DNA replication, gene expression
  • Basics of gene engineering
  • Methods for studying cell death
  • Cell cycle and mitosis
  • Human karyotype and chromozomal aberrations
  • Genetic disorders - autosomal and gonosomal inheritance
  • PCR and diagnostics of pathogenic bacteria
  • Molecular diagnostics of cancer
Literature
    required literature
  • Aktuální protokoly ke cvičením jsou dostupné v elektronické podobě na stránkách informačního systému MU (ISu): Studijní materiály předmětu BLMB011c
Teaching methods
laboratory practice, class discussion
Assessment methods
Practical classes are mandatory.
Requirements for course completion: full attendance in the practices (1 absence is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; activity in the practices.
In case of a student’s late arrival to the practice or poor activity at the practice, the student must write an essay of 2 pages on a given topic. Same for the case of two unexcused absences in practices. Find the instructions for essay writing in the Course-related instructions in the IS, bring the essay printed to the teacher who specified the topic and be ready to answer the teacher's questions regarding the topic.
In case of 3 absences (or more than 3 absences all excused by the Office for Studies within five days from the beginning of the absence and introduced to the Information System), it is addressed individually, and course-unit credit is awarded only after the proof of the student's knowledge of the entire semester topics from practices in a Credit test. Information about Credit test to be found in Interactive syllabus of the course.
Course-unit credit is not awarded in case of 4 or more unexcused absences.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
Teacher's information
https://is.muni.cz/auth/el/med/podzim2024/BLMB011c/index.qwarp
Follow the Interactive syllabus of the course: https://is.muni.cz/auth/el/med/podzim2024/BLMB011c/index.qwarp
The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.
  • Enrolment Statistics (recent)
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