VLBC0321p Biochemistry I - lecture

Faculty of Medicine
autumn 2024
Extent and Intensity
3/0/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
Mgr. Jiří Slanina, Ph.D. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Mgr. Marie Tomandlová, Ph.D. (lecturer)
Mgr. Jana Gregorová, Ph.D. (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
VLBI0222p Biology II-lect. && VLBF011p Biophysics-lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to introduce students to the basic metabolic pathways and processes occurring at the cellular level in the human organism.
Learning outcomes
Upon completion of the course, student will be able to:
- understand the meaning of basic chemical concepts and apply them in describing biochemical processes in an organism.
- discuss the properties and functions of enzymes.
- describe the basic catabolic and anabolic pathways of cellular metabolism of carbohydrates, lipids, and proteins.
- characterize the principles of energy acquisition, utilization, and storage in the cell.
- explain protein synthesis, beginning with the processes of replication and transcription, including posttranslational modifications.
- understand the relationship between the structure and function of proteins, e.g. haemoglobin, collagen, elastin.
- characterize the causes of some diseases at the molecular level.
Syllabus
  • Electrolytes, ion activity. Weak acids/bases. Buffers. Bioenergetics, Gibbs energy, High-energy compounds. Redox reaction.
  • Enzymes. Characteristic features of biocatalysis. Cofactors.
  • Metabolism of nutrients. Citrate cycle. Respiratory chain. Oxidative phosphorylation.
  • Carbohydrates. Carbohydrates in nutrition. Carbohydrate digestion. Basic features of glucose metabolism. Glucose transport into cells. Glycolysis. Gluconeogenesis.
  • Glycogen. Metabolism of fructose, galactose, lactose. The pentose phosphate pathway. Glycosaminoglycans, proteoglycans.
  • Lipids and fatty acids. Surfactants. Lipophilic vitamins. Lipids in nutrition. Lipid digestion. Fatty acid metabolism. PUFA synthesis. Metabolism of ketone bodies.
  • Metabolism of triacylglycerols, glycerophospholipids, sphingolipids. Lipoperoxidation. Biosynthesis and importance of eicosanoids. Metabolism of cholesterol, bile acids. Biosynthesis of calciols.
  • Amino acids. Peptides. Structure of proteins, myosin, collagen, immunoglobulins. Hemoglobin, myoglobin. Hemoglobinopathies.
  • Digestion and intracellular degradation of proteins. General features in catabolism of amino acids. Ammonia metabolism and toxicity.
  • Catabolism of amino acids, congenital disorders of amino acid metabolism. Synthesis of non-essential amino acids.
  • Conversions of amino acids into low molecular weight products. Heme synthesis, porphyria.
  • Metabolism of purine/pyrimidine nucleotides.
  • Structure of DNA, RNA. Replication and corrections. Transcription and its regulation, post-transcriptional modifications.
  • Protein synthesis. Folding, transport and sorting of proteins in the cell. Post-translational modification.
  • Biosynthesis of collagen, elastin.
Literature
    required literature
  • MURRAY, Robert K., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY, Victor W. RODWELL and P. Anthony WEIL. Harperova ilustrovaná biochemie. Translated by Bohuslav Matouš. Páté české vydání, prv. Praha: Galén, 2012, xii, 730. ISBN 9788072629077. info
    recommended literature
  • TOMANDL, Josef, Jiří DOSTÁL, Hana PAULOVÁ and Eva TÁBORSKÁ. Základy lékařské chemie a biochemie (Fundamentals of medical chemistry and biochemistry). 5. dotisk 1. vyd. Brno: Masarykova univerzita, 2023, 212 pp. ISBN 978-80-210-6973-2. info
  • LIEBERMAN, Michael and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 5th edition. Philadelphia: Wolters Kluwer, 2018, xii, 1051. ISBN 9781496387721. info
  • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Barevný atlas biochemie. Translated by Vladimír Benda - Martin Vejražka - Jiří Jonák. 1. české vydání. Praha: Grada, 2012, xiv, 498. ISBN 9788024729770. info
  • VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Úvod do všeobecnej a klinicky aplikovanej biochémie. Edited by Jozef Čársky. Prvé slovenské vydanie. Bratislava: Balneotherma s.r.o., 2015, 669 stran. ISBN 9788097015688. info
Teaching methods
Lectures.
Assessment methods
Credit, no knowledge testing.
The course is followed in the 4th semester by Biochemistry II. The content of Biochemistry I is included in the Biochemistry II exam.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Information on the extent and intensity of the course: 45.
The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.
  • Enrolment Statistics (recent)
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