aVLMB031 Modern Imaging and Analytical Methods in Cellular Biology and Immunology

Faculty of Medicine
autumn 2024
Extent and Intensity
0/0.7/.3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Kamila Bendíčková, Ph.D. (lecturer)
Mgr. Jan Frič, Ph.D., MHA (lecturer)
Mgr. Marcela Hortová Kohoutková, Ph.D. (lecturer)
Mgr. Petra Lázničková, Ph.D. (lecturer)
Bc. Gabriela Blažková (seminar tutor)
Mgr. Veronika Bosáková, Ph.D. (seminar tutor)
Mgr. Filip Kafka (seminar tutor)
Ioanna Papatheodorou, MSc (seminar tutor)
Mgr. Zuzana Tomášiková (seminar tutor)
Guaranteed by
Mgr. Jan Frič, Ph.D., MHA
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Mgr. Zuzana Tomášiková
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine
Timetable
Fri 1. 11. 9:00–15:00 Virtuální místnost LF/Virtual room LF, Fri 15. 11. 9:00–15:00 A 1.08
Prerequisites
aVLBI0222p Medical Biology II - lect. || aZLBI0222p Biology II-lect.
Students need to have cell biology knowledge at the level of 1st year of university, proven with an exam (aVLBI0222p, aZLBI0222p or Bi1700en).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 40 student(s).
Current registration and enrolment status: enrolled: 8/40, only registered: 0/40
fields of study / plans the course is directly associated with
Course objectives
Upon completing the course, the student will gain an overview of currently used methods for imaging and analyzing cells and molecules and will practically familiarize themselves with some of their applications. They will visit laboratories and become acquainted with instruments for visualizing biomolecules in cells and tissues (cell imaging), such as fluorescent microscopes with confocal imaging, flow cytometry, and other imaging techniques. The student will understand the principles and applications of these methods, gain a deeper understanding of modern biological object studies, an idea of the use of these methods in biomedical research and modern medicine, and will be capable of basic interpretation of the results obtained by these methods.
Learning outcomes
The student will understand the principles and potential applications of the discussed methods, gain a better understanding of modern studies of biological objects, acquire an idea of the use of these methods in biomedical research and modern medicine, and be capable of basic interpretation of the results obtained by these methods.
Syllabus
  • ** All lessons are held in English **
  • Friday, November 1, 2024:
  • Online lectures from 9:00 AM to 3:00 PM (MS Teams).
  • Lectures will be recorded and made available in the study materials.
  • Topics:
  • fluorescent labeling and confocal microscopy,
  • principles of flow cytometry and its applications,
  • cell sorting using flow cytometry,
  • advanced cytometric techniques (spectral and mass cytometry),
  • advanced microscopy techniques,
  • other methods for the analysis and visualization of biomolecules.
  • Friday, November 15, 2024 - Mandatory attendance:
  • Meeting at the Biology Park (near the University Campus Bohunice)
  • Morning session 9:00 AM - 11:15 AM:
  • Tour of the laboratory with demonstrations of the discussed methods directly in the lab.
  • Afternoon session 12:00 PM - 1:45 PM:
  • Application of imaging and analytical methods in biomedical research.
  • Final test 2:00 PM:
  • 2 open-ended questions from each discussed method covering the basic principles of the methods.
  • Contact person: 484175@mail.muni.cz; zuzana.tomasikova@fnusa.cz
Teaching methods
seminar, lab demonstration
Assessment methods
Course completion requires full attendance and passing a final written test (10 questions about the basic principles of demonstrated methods).
Attendance at an online seminar is highly recommended; in-person seminars require full attendance.
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 15.
Teacher's information
https://is.muni.cz/auth/el/med/podzim2024/VLMB011/?strpo=250?info=1
The teaching takes place in blocks on two Fridays. First online meeting: November 1st 2024 and second in-person meeting November 15th 2024.
The course is also listed under the following terms Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2022, autumn 2023.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/med/autumn2024/aVLMB031