B7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2001
Extent and Intensity
3/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 1999, Autumn 2000.
  • Enrolment Statistics (recent)
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