Course Information

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2024

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
Mgr. Michal Vašina, Ph.D. (lecturer)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Wed 16:00–17:50 B11/335

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Microbiology (programme PřF, N-MIK)

Course objectives

The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.

The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.

The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.

Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry.

Learning outcomes

The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences

During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection

At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry.

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Advanced Molecular Biology Tools
Protein Expression Technologies
Introduction to Synthetic Biology
Protein Engineering
Microfludics - Lab on a Chip
Artificial Intelligence in Life Sciences
TECHNOLOGICAL LECTURES:
Molecular Biotechnology Industry
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture
Environmental Molecular Biotechnology
(the order of courses can be changed)

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).

Assessment methods

Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.

In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.

The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F.

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2023

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

Timetable

Wed 16:00–17:50 B11/335

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Microbiology (programme PřF, N-MIK)

Course objectives

Learning outcomes

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Advanced Molecular Biology Tools
Protein Expression Technologies
Introduction to Synthetic Biology
Protein Engineering
Microfludics - Lab on a Chip
Artificial Intelligence in Life Sciences
TECHNOLOGICAL LECTURES:
Molecular Biotechnology Industry
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture
Environmental Molecular Biotechnology
(the order of courses can be changed)

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2022

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

Timetable

Wed 16:00–17:50 B11/335

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Microbiology (programme PřF, N-MIK)

Course objectives

Learning outcomes

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Advanced Molecular Biology Tools
Protein Expression Technologies
Introduction to Synthetic Biology
Protein Engineering
Microfludics - Lab on a Chip
Artificial Intelligence in Life Sciences
TECHNOLOGICAL LECTURES:
Molecular Biotechnology Industry
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture
Environmental Molecular Biotechnology
(the order of courses can be changed)

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430 Molecular biotechnology

Faculty of Science
autumn 2021

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

Timetable

Wed 11:00–12:50 B11/305

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Microbiology (programme PřF, N-MIK)

Course objectives

Learning outcomes

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Advanced Molecular Biology Tools
Protein Expression Technologies
Introduction to Synthetic Biology
Protein Engineering
Microfludics - Lab on a Chip
Artificial Intelligence in Life Sciences
TECHNOLOGICAL LECTURES:
Molecular Biotechnology Industry
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture
Environmental Molecular Biotechnology
(the order of courses can be changed)

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2020

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D., MBA (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Ing. Andrea Smith, PhD. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)

Guaranteed by

Timetable

Wed 16:00–17:50 prace doma

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Microbiology (programme PřF, N-MIK)

Course objectives

Learning outcomes

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Basic Molecular Biology Tools
Protein Expression Technologies
Introduction to Synthetic Biology
Protein Engineering
Microfludics - Lab on a Chip
Artificial Intelligence in Life Sciences
TECHNOLOGICAL LECTURES:
Molecular Biotechnology Industry
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture
Environmental Molecular Biotechnology
(the order of courses can be changed)

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Teaching will be organized online in the autumn 2020 in the MS Teams environment.

During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2019

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

Timetable

Thu 14:00–15:50 B11/335

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Microbiology (programme PřF, N-MIK)

Course objectives

Learning outcomes

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Basic Molecular Biology Tools
Protein Expression Technologies
Introduction to Synthetic Biology
Protein Engineering
Microfludics - Lab on a Chip
Artificial Intelligence in Life Sciences
TECHNOLOGICAL LECTURES:
Molecular Biotechnology Industry
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture
Environmental Molecular Biotechnology
(the order of courses can be changed)

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.

In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.

The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F.

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2018

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. Andrea Schenkmayerová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)

Guaranteed by

Timetable

Mon 1. 10. to Fri 14. 12. Thu 10:00–11:50 B11/335

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.

Learning outcomes

Student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA;
- basic methods in synthetic biology;
- protein engineering;
- microfluidics and "lab on chip" technologies;

During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics;
- long-term sustainable industries;
- modern agriculture and biofuels;
- environmental protection;

At the end of this course the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.

Syllabus

Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
Basic Molecular Biology Tools
Metabolic Engineering
Protein Engineering
Microfludics - Lab on a Chip
TECHNOLOGICAL LECTURES:
Biofuels
Molecular Biotechnology Industry
Environmental Molecular Biotechnology
Molecular Biotechnology in Medicine I.
Molecular Biotechnology in Medicine II.
Molecular Biotechnology in Agriculture

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.

In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.

The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F).

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
autumn 2017

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Ing. Andrea Schenkmayerová, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Lukáš Chrást (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)

Guaranteed by

Timetable

Mon 18. 9. to Fri 15. 12. Thu 16:00–17:50 D29/252-RCX1

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

Learning outcomes

Syllabus

1. Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
2. Basic Molecular Biology Tools
3. Introduction to Synthetic Biology
4. Protein Engineering
5. Microfludics - Lab on a Chip
TECHNOLOGICAL LECTURES:
6. Biofuels
7. Molecular Biotechnology Industry
8. Environmental Molecular Biotechnology
9. Molecular Biotechnology in Medicine I.
10. Molecular Biotechnology in Medicine II.
11. Molecular Biotechnology in Agriculture

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.

In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.

The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F).

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2016

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. Táňa Koudeláková, Ph.D. (lecturer)
Mgr. Lukáš Chrást (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)

Guaranteed by

Timetable

Mon 19. 9. to Sun 18. 12. Thu 15:00–16:50 C05/114

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

Syllabus

1. Opening motivation lecture / Organization of the course
2. Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
3. Molecular Biology Tools
4. Protein Engineering
5. Microfludics - Lab on a Chip
TECHNOLOGICAL LECTURES:
6. Biofuels
7. Environmental molecular biotechnology
8. Molecular biotechnology industry
9. Molecular biotechnology in medicine I.
10. Molecular biotechnology in medicine II.
11. Molecular Biotechnology in Agriculture

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Short written tests will be prepared at the beginning of lectures No. 4, 6, 8 and 10. Each short written test will contain ten questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.

In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.

The student receives a score according to the following criteria (success in test questions / evaluation:% 100-91 / A; 90-76% / B, 75-61% / C, 60-46% / D, 45-31% / E; 30 -0% / F).

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2015

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Táňa Koudeláková, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)

Guaranteed by

Timetable

Thu 12:00–13:50 B11/235

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

Syllabus

1. Opening motivation lecture / Organization of the course
2. Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
3. Molecular Biology Tools
4. Protein Engineering
5. Metabolic Engineering I.
6. Metabolic Engineering II.
7. Microfludics - Lab on a Chip
TECHNOLOGICAL LECTURES:
8. Molecular Biotechnology in Agriculture
9. Molecular biotechnology industry
10. Molecular biotechnology in medicine I.
11. Molecular biotechnology in medicine II.
12. Environmental molecular biotechnology

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2014

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Koen Beerens, PhD. (lecturer)
Mgr. Martina Damborská (assistant)

Guaranteed by

Timetable

Mon 16:00–17:50 B11/306

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

Syllabus

1. Opening lecture / Organization of the course
2. Introduction to Molecular Biotechnology
METHODOLOGICAL LECTURES:
3. Protein Engineering
4. Metabolic Engineering I.
5. Metabolic Engineering II.
6. Microfludics - Lab on a Chip
TECHNOLOGICAL LECTURES:
7. Molecular Biotechnology in Agriculture
8. Molecular biotechnology industry
9. Molecular biotechnology in medicine I.
10. Molecular biotechnology in medicine II.
11. Molecular biotechnology in medicine III.
12. Environmental molecular biotechnology

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2013

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (lecturer)
Koen Beerens, PhD. (lecturer)
Mgr. Martina Damborská (assistant)

Guaranteed by

Timetable

Mon 16. 9. to Fri 6. 12. Mon 9:00–10:50 B11/235

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

Syllabus

1. Opening lecture / Organization of the course
2. Introduction to Molecular Biotechnology
3. Protein Engineering
4. Metabolic Engineering I.
5. Metabolic Engineering II.
6. Molecular Biotechnology in Agriculture
7. Molecular biotechnology industry
8. Molecular biotechnology in medicine I.
9. Molecular biotechnology in medicine II.
10. Molecular biotechnology in medicine III.

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2012

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)

Guaranteed by

Timetable

Thu 10:00–11:50 B11/235

Prerequisites

Basic knowledge of biochemistry, microbiology, molecular biology and genetics.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

The course of molecular biotechnology is focused on the specific aspects of modern biotechnology covering the latest innovations and development of therapeutics, vaccines, biotransformations, transgenic plants and animals and the role of these modern technologies in sustainable living. The number of examples illustrates the latest principles and applications of recombinant DNA technology in up to date industrial, agricultural, pharmaceutical, and biomedical applications.

In this course, students learn about the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will acquire the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research and development or biotechnology industry.

Syllabus

1. Opening lecture / Organization of the course
2. Introduction to Molecular Biotechnology
3. Protein Engineering
4. Metabolic Engineering I.
5. Metabolic Engineering II.
6. Molecular biotechnology industry
7. Molecular biotechnology in medicine I.
8. Molecular biotechnology in medicine II.
9. Molecular Biotechnology in Agriculture
10. Molecular Biotechnology and Environmental Protection

Literature

An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009, xix, 604. ISBN 9780854041251. info

Teaching methods

During lecturing, the powerpoint presentations are used, pdf materials for printing and notices are available, prepared are short written tests at the beginning of selected lectures and short exercises and discussions during lectures.

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2011

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 12:00–13:50 Bpt,01013

Prerequisites

The course is destined for students interested in practical use of recombinant DNA technology in molecular biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biology (Bi4020) and General Microbiology (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

At the end of this course, the students should be able to explain principles of the use of recombinant DNA technology for gene manipulation and construction of genetically modified organisms producing commercial products. Using the acquired data they will be able to understand the different cloning systems used and their use in preparation of different commercial products. Based on the obtained knowledge, they will be able to assess and evaluate new genetically modified organisms.

Syllabus

Relationship between chemical production and biotechnologies.
Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
Recombinant DNA technology. Plasmid cloning vectors and their use.
Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
Vectors for cloning large fragments of DNA.
Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
Increasing of protein stability. Fusion proteins.
Translation expression vectors. DNA integration into host chromosome.
Heterologous protein production in eukaryotic cells.
Directed mutagenesis and protein engineering.
Microbial molecular biotechnology. Molecular diagnostics.
Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
Production of small biological molecules, antibiotics and biopolymers.
Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.

Literature

ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info

Teaching methods

Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.

Assessment methods

The course is closed with a test and a spoken exam. The test contains 60 questions, which must be answered briefly. Each student who has correctly answered 40 questions is qualified for the spoken exam. The student answers 2 to 3 questions in the exam. The overall result of the exam is evaluated by the examiner.

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2010

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 8:00–9:50 Bpt,01013

Prerequisites

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies.
Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
Recombinant DNA technology. Plasmid cloning vectors and their use.
Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
Vectors for cloning large fragments of DNA.
Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
Increasing of protein stability. Fusion proteins.
Translation expression vectors. DNA integration into host chromosome.
Heterologous protein production in eukaryotic cells.
Directed mutagenesis and protein engineering.
Microbial molecular biotechnology. Molecular diagnostics.
Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
Production of small biological molecules, antibiotics and biopolymers.
Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.

Literature

ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info

Teaching methods

Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2009

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Wed 15:00–16:50 Bpt,01013

Prerequisites

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies.
Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
Recombinant DNA technology. Plasmid cloning vectors and their use.
Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
Vectors for cloning large fragments of DNA.
Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
Increasing of protein stability. Fusion proteins.
Translation expression vectors. DNA integration into host chromosome.
Heterologous protein production in eukaryotic cells.
Directed mutagenesis and protein engineering.
Microbial molecular biotechnology. Molecular diagnostics.
Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
Production of small biological molecules, antibiotics and biopolymers.
Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.

Literature

ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info

Teaching methods

Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2008

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 11:00–12:50 Bpt,01013

Prerequisites

The course is for the students with interest about molecular biotechnology. Prerequisites are exams from Molecular biology(Bi4020) and General microbiology(Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Definition of molecular biotechnology and its emergence. Impact of recombinant DNA technology on molecular biotechnology. Procedures enabling gene transfer from one organism into an other (vectors for cloning of DNA fragments of different lengths, construction and screening of gene and genome libraries, cDNA and metagenomic libraries). Manipulation of gene expression in prokaryotes (the use of strong and regulatable promotors, expression vectors, integration vectors, vectors for construction of fusion proteins). Heterologous protein production in eukaryotic cells. Microbial molecular biotechnology and its use in molecular diagnostics, in production of small molecules, therapeutic proteins and vaccines, in bioremediation and in biomass utilisation. The next use of transgenic microorganisms, plants and animals. Regulating of GMO use. This course is supplemented by practical course Bi7430c.
At the end of this course, student should be able to explain the use of recombinant DNA technology for the construction of genetic modified organisms producing commercial products. Using aquired data he will be able to understand new genetic modified organisms.

Syllabus

Relationship between chemical production and biotechnologies.
Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
Recombinant DNA technology. Plasmid cloning vectors and their use.
Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
Vectors for cloning large fragments of DNA.
Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
Increasing of protein stability. Fusion proteins.
Translation expression vectors. DNA integration into host chromosome.
Heterologous protein production in eukaryotic cells.
Directed mutagenesis and protein engineering.
Microbial molecular biotechnology. Molecular diagnostics.
Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
Production of small biological molecules, antibiotics and biopolymers.
Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.

Literature

ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info

Assessment methods

Lectures are enclosed by test and oral exam.

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2007

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Mon 9:00–10:50 Bpt,01013

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creating and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Directed mutagenesis and protein engineering. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2006

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 14:00–15:50 Bpt,01013

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2005

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Wed 13:00–14:50 Bpt,01013

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2004

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 11:00–12:50 Bpt,01013

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creating and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Directed mutagenesis and protein engineering. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2003

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Úvod do molekulární biotechnologie

Faculty of Science
Autumn 2002

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies.
Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
Recombinant DNA technology. Plasmid cloning vectors and their use.
Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
Vectors for cloning large fragments of DNA.
Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
Increasing of protein stability. Fusion proteins.
Translation expression vectors. DNA integration into host chromosome.
Heterologous protein production in eukaryotic cells.
Directed mutagenesis and protein engineering.
Microbial molecular biotechnology. Molecular diagnostics.
Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
Production of small biological molecules, antibiotics and biopolymers.
Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.

Literature

ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info

Teaching methods

Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2010 - only for the accreditation

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies.
Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
Recombinant DNA technology. Plasmid cloning vectors and their use.
Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
Vectors for cloning large fragments of DNA.
Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
Increasing of protein stability. Fusion proteins.
Translation expression vectors. DNA integration into host chromosome.
Heterologous protein production in eukaryotic cells.
Directed mutagenesis and protein engineering.
Microbial molecular biotechnology. Molecular diagnostics.
Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
Production of small biological molecules, antibiotics and biopolymers.
Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.

Literature

ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info

Teaching methods

Presentations in PowerPoint are used in the instruction. The course is followed up by a practical laboratory course.

Assessment methods

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2007 - for the purpose of the accreditation

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)

Guaranteed by

doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

General Biology (programme PřF, M-BI, specialization Microbiology)

Course objectives

Syllabus

Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.

Literature

Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.

Assessment methods (in Czech)

Předmět je ukončen zkouškou

Language of instruction

Czech

Follow-Up Courses

Bi9080 Technical processes in biotechnology

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

Bi7430c Molecular biotechnology - practical course
NOW(Bi7430)

The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Enrolment Statistics (recent)

Course Information

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Úvod do molekulární biotechnologie

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

Bi7430 Molecular biotechnology

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