Course Information

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
Mgr. Bc. Alan Strunga (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
PharmDr. Bc. Josef Mašek, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Wed 9:00–10:50 C13/332, D. Kovář, Z. Prokop, M. Vašina
Bi7430c/02: Wed 14:00–15:50 C13/332, D. Kovář, Z. Prokop, M. Vašina

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Microbiology (programme PřF, N-MIK)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

At the end of the exercise, students will know a number of practical procedures of molecular biotechnology, e.g. primer design, DNA cleavage with restriction enzymes, transformation of bacterial cells, methods of fermentation cultivation of E. coli, use of recombinant technologies in biosensing and removal of dangerous substances, development of recombinant therapeutics and vaccines. The course will allow students to deepen their theoretical knowledge acquired in the Molecular Biotechnology lectures.

Learning outcomes

The exercise provides a combination of practical skills taught by PřF MUNI and VÚVeL experts. Students will get to know the currently used methods as well as the principle and operation of modern laboratory equipment. The subject also aims to strengthen students' communication skills through discussions with experts in the given field, it will also help the development of students' stylistic skills during the independent preparation of essays and protocols from completed exercises. The use of study materials in English will support the ability to work with professional texts, including consolidating knowledge of English terminology used in this field.

Syllabus

• Advanced Molecular Biology Tools
• Protein Expression Technologies
• Microfluidics, Lab on a Chip
• Drug delivery systems and recombinant vaccines
• Development of recombinant protein therapeutics
• Enzymatic assay for determination of environmental pollutants
• Basics of biochemical data analysis

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample or dataset.
The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice.
Students will receive ELECTRONIC MATERIALS for each topic (e.g., methodologies, methodological articles, video tutorials)

Assessment methods

Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
Ing. Jan Kotouček, Ph.D. (seminar tutor)
Mgr. Bc. Alan Strunga (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Wed 9:00–10:50 C13/332, D. Kovář, Z. Prokop, M. Vašina
Bi7430c/02: Wed 13:00–14:50 C13/332, D. Kovář, Z. Prokop, M. Vašina

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Microbiology (programme PřF, N-MIK)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The exercise provides a combination of practical skills taught by experts from academia (PřF MU and VÚVeL) and the corporate environment (biotechnology companies Enantis, NEXARS). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides the students with an interesting combination of practical knowledge under the guidance of experts from academia (MU and VRI) and business environment (biotechnology companies Enantis, NEXARS). The student will become trained in modern methods:

Design of recombinant systems

Fermentation of recombinant microorganisms

Preparation of enzymatic biosensor

Microfluidics, lab on a chip

Biocatalytic preparation of pharmaceutical precursor

Preparation and transformation of liposomes

Analysis of liposomes

Syllabus

• Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• Microfluidics, lab on a chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.
The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice.
Students will receive ELECTRONIC MATERIALS for each topic (e.g., methodologies, methodological articles, video tutorials)

Assessment methods

Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
Ing. Jan Kotouček, Ph.D. (seminar tutor)
Mgr. Bc. Alan Strunga (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Tue 10:00–11:50 C13/208, D. Kovář, Z. Prokop, M. Vašina
Bi7430c/02: Tue 14:00–15:50 C13/208, D. Kovář, Z. Prokop, M. Vašina

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Microbiology (programme PřF, N-MIK)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The exercise provides a combination of practical skills taught by experts from academia (PřF MU and VÚVeL) and the corporate environment (biotechnology companies Enantis, NEXARS). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides the students with an interesting combination of practical knowledge under the guidance of experts from academia (MU and VRI) and business environment (biotechnology companies Enantis, NEXARS). The student will become trained in modern methods:

Design of recombinant systems

Fermentation of recombinant microorganisms

Preparation of enzymatic biosensor

Microfluidics, lab on a chip

Biocatalytic preparation of pharmaceutical precursor

Preparation and transformation of liposomes

Analysis of liposomes

Syllabus

• Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• Microfluidics, lab on a chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.
The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice.
Students will receive ELECTRONIC MATERIALS for each topic (e.g., methodologies, methodological articles, video tutorials)

Assessment methods

Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
PharmDr. Josef Mašek, Ph.D. (seminar tutor)
Jana Škařupová, MSc. (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Tue 10:00–11:50 Kontaktujte učitele, D. Kovář, Z. Prokop
Bi7430c/02: Tue 14:00–15:50 Kontaktujte učitele, D. Kovář, Z. Prokop

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Microbiology (programme PřF, N-MIK)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The exercise provides a combination of practical skills taught by experts from academia (PřF MU and VÚVeL) and the corporate environment (biotechnology companies Enantis, NEXARS). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides the students with an interesting combination of practical knowledge under the guidance of experts from academia (MU and VRI) and business environment (biotechnology companies Enantis, NEXARS). The student will become trained in modern methods:

Design of recombinant systems

Fermentation of recombinant microorganisms

Preparation of enzymatic biosensor

Microfluidics, lab on a chip

Biocatalytic preparation of pharmaceutical precursor

Preparation and transformation of liposomes

Analysis of liposomes

Syllabus

• Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• Microfluidics, lab on a chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.
The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice.
Students will receive ELECTRONIC MATERIALS for each topic (e.g., methodologies, methodological articles, video tutorials)

Assessment methods

Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. Andrea Smith, PhD. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
Mgr. Andrea Pařízková (seminar tutor)
PharmDr. Josef Mašek, Ph.D. (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Wed 10:00–11:50 Kontaktujte učitele, D. Kovář, Z. Prokop, A. Smith
Bi7430c/02: Wed 14:00–15:50 Kontaktujte učitele, D. Kovář, Z. Prokop, A. Smith

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430).

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Microbiology (programme PřF, N-MIK)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The exercise provides a combination of practical skills taught by experts from academia (PřF MU and VÚVeL) and the corporate environment (biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides the students with an interesting combination of practical knowledge under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). The student will become trained in modern methods:

Design of recombinant systems

Fermentation of recombinant microorganisms

Preparation of enzymatic biosensor

Microfluidics, lab on a chip

Biocatalytic preparation of pharmaceutical precursor

Preparation and transformation of liposomes

Analysis of liposomes

Syllabus

• Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• Microfluidics, lab on a chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

ORGANIZATION OF DISTANCE TEACHING
regarding to the Covid pandemic, for the autumn 2020

Students will receive ELECTRONIC MATERIALS for each topic (eg methodologies, methodological articles, video tutorials)

After the study, a SHORT WRITTEN TEST will be prepared, verifying the obtained knowledge in the given topic.

During the semester, an ON-LINE DISCUSSION (MS Teams) will be organized on current or controversial topics (eg gene editing, GMO).

Assessment methods

The evaluation will be awarded after completing all short tests on individual topics and fulfilling active participation in the online discussion.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. Andrea Smith, PhD. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Mgr. Michal Vašina, Ph.D. (seminar tutor)
Mgr. Andrea Pařízková (seminar tutor)
PharmDr. Josef Mašek, Ph.D. (seminar tutor)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Wed 10:00–11:50 Kontaktujte učitele, Z. Prokop, A. Smith
Bi7430c/02: Wed 14:00–15:50 Kontaktujte učitele, Z. Prokop, A. Smith

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20

fields of study / plans the course is directly associated with

• Microbiology (programme PřF, N-MIK)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides the students with an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). In practical exercises the student will become trained in modern methods:

Design of recombinant systems

Fermentation of recombinant microorganisms

Preparation of enzymatic biosensor

Microfluidics, lab on a chip

Biocatalytic preparation of pharmaceutical precursor

Preparation and transformation of liposomes

Analysis of liposomes

At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen the communication skills of students in a discussion with experts in the field and exercise stylistic skills of students in writing essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Syllabus

• Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• Microfluidics, lab on a chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory

Assessment methods

The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Mgr. David Kovář, Ph.D. (seminar tutor)
Ing. RNDr. Martin Marek, Ph.D., MBA (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Ing. Andrea Schenkmayerová, Ph.D. (seminar tutor)
Mgr. Martina Damborská (assistant)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Mon 17. 9. to Fri 14. 12. Wed 10:00–11:50 Kontaktujte učitele, Z. Prokop, J. Turánek
Bi7430c/02: Mon 17. 9. to Fri 14. 12. Wed 14:00–15:50 Kontaktujte učitele, Z. Prokop, J. Turánek

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides to the students an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). In practical exercises the student will become trained in modern methods:

Design of recombinant systems

Fermentation of recombinant microorganisms

Preparation of enzymatic biosensor

Preparation and testing of microfluidic chip

Biocatalytic preparation of pharmaceutical precursor

Preparation and transformation of liposomes

Analysis of liposomes

At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts in the field and exercise stylistic skills of students in writing essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Syllabus

• Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• Preparation and testing of microfluidic chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory

Assessment methods

The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Veronika Lišková, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
Mgr. Tomáš Buryška, Ph.D. (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Mon 18. 9. to Fri 15. 12. Wed 10:00–11:50 Kontaktujte učitele, L. Chrást, Z. Prokop, V. Štěpánková
Bi7430c/02: Mon 18. 9. to Fri 15. 12. Wed 14:00–15:50 Kontaktujte učitele, L. Chrást, Z. Prokop, V. Štěpánková

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Learning outcomes

The practice provides to the students an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). In practical exercises the student will become trained in modern methods:

1. Design of recombinant systems

2. Fermentation of recombinant microorganisms

3. Preparation of enzymatic biosensor

4. Preparation and testing of microfluidic chip

5. Biodegradation of environmental pollutant by recombinant bacterium

6. Biocatalytic preparation of pharmaceutical precursor

7. Preparation and transformation of liposomes

8. Analysis of liposomes

At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts in the field and exercise stylistic skills of students in writing essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Syllabus

• 1. Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• 2. Preparation and testing of microfluidic chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• 2. Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• 3. Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• 5. Biodegradation of environmental pollutant by recombinant bacterium
• engineered bacterium, synthetic metabolic pathway, biodegradation of environmental pollutant, analysis of metabolites
• 6. Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• 7. Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• 8. Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory

Assessment methods

The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Teacher's information

https://loschmidt.chemi.muni.cz/peg/courses/

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Táňa Koudeláková, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
Mgr. Tomáš Buryška, Ph.D. (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Mon 19. 9. to Sun 18. 12. Wed 10:00–11:50 Kontaktujte učitele, L. Chrást, Z. Prokop, J. Turánek
Bi7430c/02: Mon 19. 9. to Sun 18. 12. Wed 14:00–15:50 Kontaktujte učitele, L. Chrást, Z. Prokop, J. Turánek

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Syllabus

• 1. Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• 2. Preparation and testing of microfluidic chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• 2. Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• 3. Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• 5. Biodegradation of environmental pollutant by recombinant bacterium
• engineered bacterium, synthetic metabolic pathway, biodegradation of environmental pollutant, analysis of metabolites
• 6. Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• 7. Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• 8. Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory

Assessment methods

The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
Mgr. Tomáš Buryška, Ph.D. (seminar tutor)
Mgr. Veronika Lišková, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi7430c/01: Wed 10:00–11:50 Kontaktujte učitele, P. Dvořák, Z. Prokop
Bi7430c/02: Wed 13:00–14:50 Kontaktujte učitele, P. Dvořák, Z. Prokop

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Syllabus

• 1. Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• 2. Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• 3. Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• 4. Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• 5. Biodegradation of environmental pollutant by recombinant bacterium
• engineered bacterium, synthetic metabolic pathway, biodegradation of environmental pollutant, analysis of metabolites
• 6. Preparation and testing of microfluidic chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• 7. Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• 8. Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory

Assessment methods

The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Koen Beerens, PhD. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
doc. Mgr. Pavel Dvořák, Ph.D. (seminar tutor)
Mgr. Tomáš Buryška, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Wed 15:00–16:50 C13/208

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.

Syllabus

• 1. Design of recombinant systems
• software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
• 2. Fermentation of recombinant microorganisms
• fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
• 3. Preparation of enzymatic biosensor
• co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
• 4. Biocatalytic preparation of pharmaceutical precursor
• biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
• 5. Biodegradation of environmental pollutant by recombinant bacterium
• engineered bacterium, synthetic metabolic pathway, biodegradation of environmental pollutant, analysis of metabolites
• 6. Preparation and testing of microfluidic chip
• fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
• 7. Preparation and transformation of liposoms
• preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
• 8. Analysis of liposoms
• single particle tracking analysis by using Nanosight 500, transmission electron microscopy

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory

Assessment methods

The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
Course is no more offered.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Koen Beerens, PhD. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
Mgr. Hana Moskalíková (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Mon 14:00–15:50 C03/208

Prerequisites

NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Bi7430 Molecular Biotechnology lecture.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

At the end of the course students will have practical experience with range of procedures of molecular biotechnology methods, e.g. design of primers, DNA cleavage using restrictive enzymes, bacterial cell transformation, fermentation cultivation of E. coli, recombinant protein purification and its use in biocatalysis and biosensing.

Syllabus

• BLOK A
• 1. Preparation and transformation of liposoms
• (preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer)
• 2. Analysis of liposoms
• (Single particle tracking analysis by using Nanosight 500, transmission electron microscopy)
• BLOK B
• 3. Preparation of recombinant E. coli
• (Clone Manager, design of primers, restriction cleavage, cloning, transformation)
• 4. Cultivation of recombinant E. coli
• (fermentation of E. coli biomass and expression of recombinant protein)
• 5. Purification of recombinant protein
• (disintegration of biomass, purification of recombinant enzyme, gel electrophoresis)
• 6. Preparation of biosensor
• (co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte)
• 7. Biocatalytic preparation of pharmaceutical precursor
• (biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme)

Literature

• An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
• GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info

Teaching methods

In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample. The results of experiments are evaluated by each student in separate protocols (according to the outline: introduction, aim, material and methods, results, discussion, conclusion). Each student describes analyses, evaluates and discusses the results individually and independently.

Assessment methods

Students will obtain positive assessment of the practice for active participation in exercises and preparation of protocols from each exercise.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
Course is no more offered.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
doc. Mgr. Pavel Dvořák, Ph.D. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)

Guaranteed by

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is destined for the students with practical interest in selected laboratory procedures of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

At the end of the course students should be able to explain principles and utilize selected procedures of molecular biotechnology, e.g. methods of E. coli cultivation, microisolation of plasmid DNA, methods of bacterial cell transformation and selection of transformants carrying both non-recombinant and recombinant plasmid DNA, DNA cleavage using restrictases, and estimation of restriction fragments. During the course the students will learn the Southern blot, dot blot, DNA probe preparation, and DNA/DNA hybridisation including immunological detection of hybridisation products.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Teaching methods

During the laboratory course, each student works independently and processes his own sample. They must evaluate the results of their experiments in protocols. The protocols (4 in number) are elaborated in the form of a poster according to the following scheme: Introduction, Aim of work, Material and methods, Results, Discussion, Conclusion. In the protocol each student describes, compares, analyses, evaluates, and discusses his or her own results.

Assessment methods

The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course and for the elaboration of protocols (4).

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
Course is no more offered.
The course is taught: every week.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable of Seminar Groups

Bi7430c/01: Mon 8:00–11:50 Bpb,02012, B. Rittich, A. Španová, M. Vojtíšková
Bi7430c/02: Mon 12:00–15:50 Bpb,02012, B. Rittich, A. Španová, M. Vojtíšková

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is destined for the students with practical interest in selected laboratory procedures of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

At the end of the course students should be able to explain principles and utilize selected procedures of molecular biotechnology, e.g. methods of E. coli cultivation, microisolation of plasmid DNA, methods of bacterial cell transformation and selection of transformants carrying both non-recombinant and recombinant plasmid DNA, DNA cleavage using restrictases, and estimation of restriction fragments. During the course the students will learn the Southern blot, dot blot, DNA probe preparation, and DNA/DNA hybridisation including immunological detection of hybridisation products.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Teaching methods

During the laboratory course, each student works independently and processes his own sample. They must evaluate the results of their experiments in protocols. The protocols (4 in number) are elaborated in the form of a poster according to the following scheme: Introduction, Aim of work, Material and methods, Results, Discussion, Conclusion. In the protocol each student describes, compares, analyses, evaluates, and discusses his or her own results.

Assessment methods

The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course and for the elaboration of protocols (4).

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable of Seminar Groups

Bi7430c/01: Mon 8:00–11:50 Bpb,02012, B. Rittich, A. Španová, M. Vojtíšková
Bi7430c/02: Mon 12:00–15:50 Bpb,02012, B. Rittich, A. Španová, M. Vojtíšková

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is destined for the students with practical interest in selected laboratory procedures of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

At the end of the course students should be able to explain principles and utilize selected procedures of molecular biotechnology, e.g. methods of E. coli cultivation, microisolation of plasmid DNA, methods of bacterial cell transformation and selection of transformants carrying both non-recombinant and recombinant plasmid DNA, DNA cleavage using restrictases, and estimation of restriction fragments. During the course the students will learn the Southern blot, dot blot, DNA probe preparation, and DNA/DNA hybridisation including immunological detection of hybridisation products.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Teaching methods

During the laboratory course, each student works independently and processes his own sample. They must evaluate the results of their experiments in protocols. The protocols (4 in number) are elaborated in the form of a poster according to the following scheme: Introduction, Aim of work, Material and methods, Results, Discussion, Conclusion. In the protocol each student describes, compares, analyses, evaluates, and discusses his or her own results.

Assessment methods

The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course and for the elaboration of protocols (4).

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable of Seminar Groups

Bi7430c/01: Mon 8:00–11:50 Bpb,02012, B. Rittich, A. Španová, M. Vojtíšková
Bi7430c/02: Mon 12:00–15:50 Bpb,02012, B. Rittich, A. Španová, M. Vojtíšková

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is destined for the students with practical interest in selected laboratory procedures of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

At the end of the course students should be able to explain principles and utilize selected procedures of molecular biotechnology, e.g. methods of E. coli cultivation, microisolation of plasmid DNA, methods of bacterial cell transformation and selection of transformants carrying both non-recombinant and recombinant plasmid DNA, DNA cleavage using restrictases, and estimation of restriction fragments. During the course the students will learn the Southern blot, dot blot, DNA probe preparation, and DNA/DNA hybridisation including immunological detection of hybridisation products.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Teaching methods

During the laboratory course, each student works independently and processes his own sample. They must evaluate the results of their experiments in protocols. The protocols (4 in number) are elaborated in the form of a poster according to the following scheme: Introduction, Aim of work, Material and methods, Results, Discussion, Conclusion. In the protocol each student describes, compares, analyses, evaluates, and discusses his or her own results.

Assessment methods

The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course and for the elaboration of protocols (4).

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable

Thu 8:00–11:50 Bpb,02012

• Timetable of Seminar Groups:

Bi7430c/01: No timetable has been entered into IS. B. Rittich, A. Španová, M. Vojtíšková

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is for the students with practical interest in selected laboratory methods of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in molecular biotechnical laboratory. Word with GMO. Cultivation of bacterial cells E. coli carrying recombinant and nonrecombinant plasmid pUC19 DNA. Microisolation of plasmid DNA and recombinant plasmid DNA estimation of their lenghts and the lenght of insert.Transfer of reombinant and nonrecombinant plasmid DNA into bacterial cells E. coli JM109. Plating and selection of transformants. Preparation of DNA probe labelled with digoxigenine, performance of DNA/DNA hybridization (dot blot and Southern blot) and immunological detection of hybridization products. Evaluation of results. The practical course is supplemented by course Bi7430.
At the end of this course, each student should be able to known some procedures of molecular biotechnology. There are included methods of microisolation of plasmid DNA, estimation of insert length,, transformation of plastid DNA into bacterial cells and selection of transformants. In the next part of the course students will prepare DNA probe and performe DNA/DNA hybridisation including imunological detection of hybridisation product.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Assessment methods

Training is enclosed by a test.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable

Thu 8:00–11:50 Bpb,02012

Prerequisites (in Czech)

NOW( Bi7430 Molecular biotechnology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.

Syllabus

• 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.

Literature

• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable

Thu 8:00–11:50 Bpb,02012

Prerequisites (in Czech)

NOW( Bi7430 Molecular biotechnology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.

Syllabus

• 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.

Literature

• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
doc. RNDr. Alena Španová, CSc. (seminar tutor)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable

Thu 8:00–11:50 Bpb,02012

Prerequisites (in Czech)

NOW( Bi7430 Molecular biotechnology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.

Syllabus

• 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.

Literature

• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
doc. RNDr. Alena Španová, CSc. (seminar tutor)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Timetable

Thu 8:00–11:50 Bpb,02012

Prerequisites (in Czech)

NOW( Bi7430 Molecular biotechnology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.

Syllabus

• 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.

Literature

• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
doc. RNDr. Alena Španová, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Prerequisites (in Czech)

NOW( Bi7430 Molecular biotechnology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.

Syllabus

• 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.

Literature

• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is destined for the students with practical interest in selected laboratory procedures of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

At the end of the course students should be able to explain principles and utilize selected procedures of molecular biotechnology, e.g. methods of E. coli cultivation, microisolation of plasmid DNA, methods of bacterial cell transformation and selection of transformants carrying both non-recombinant and recombinant plasmid DNA, DNA cleavage using restrictases, and estimation of restriction fragments. During the course the students will learn the Southern blot, dot blot, DNA probe preparation, and DNA/DNA hybridisation including immunological detection of hybridisation products.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Teaching methods

During the laboratory course, each student works independently and processes his own sample. They must evaluate the results of their experiments in protocols. The protocols (4 in number) are elaborated in the form of a poster according to the following scheme: Introduction, Aim of work, Material and methods, Results, Discussion, Conclusion. In the protocol each student describes, compares, analyses, evaluates, and discusses his or her own results.

Assessment methods

The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course and for the elaboration of protocols (4).

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Prerequisites

NOW( Bi7430 Molecular biotechnology )
The course is destined for the students with practical interest in selected laboratory procedures of molecular biotechnology.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

At the end of the course students should be able to explain principles and utilize selected procedures of molecular biotechnology, e.g. methods of E. coli cultivation, microisolation of plasmid DNA, methods of bacterial cell transformation and selection of transformants carrying both non-recombinant and recombinant plasmid DNA, DNA cleavage using restrictases, and estimation of restriction fragments. During the course the students will learn the Southern blot, dot blot, DNA probe preparation, and DNA/DNA hybridisation including immunological detection of hybridisation products.

Syllabus

• 1. Safety of work in an molecular biotechnology laboratory. Work withGMO.
• 2. Cultivation of bacterial E. coli JM109, E. coli JM 109 (pUC19) a E. coli JM109 (pUC19::dim1) .
• 3. Microisolation of plasmid DNA pUC19 and recombinant plasmid DNA pUC19::dim1.
• 4. Estimation of plasmid DNA isolation using agarose gel electrophoresis.
• 5. Transfer of recombinant and nonrecombinant plasmid DNA into competent bacterial cells E. coli JM109
• 6. Plating and selection of transformants carrying recombinant and nonrecombinant plasmid DNA.
• 7. Evaluation of transformation.
• 8.Cleavage of plasmid DNAs with restrictase EcoRI (linearisation) and BamHI.
• 9. Gel electrophoresis of DNA and estimation of restriction fragment lenghts using lambda/HindIII standards.
• 10. Southern blot. Preparation of DNA for dot blot hybridisation.
• 11. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine.
• 12. DNA/DNA hybridization at high stringent conditions.
• 13. Immunological detection of hybridization products.
• 14. Evaluation of results of hybridization.Test.

Literature

• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.

Teaching methods

During the laboratory course, each student works independently and processes his own sample. They must evaluate the results of their experiments in protocols. The protocols (4 in number) are elaborated in the form of a poster according to the following scheme: Introduction, Aim of work, Material and methods, Results, Discussion, Conclusion. In the protocol each student describes, compares, analyses, evaluates, and discusses his or her own results.

Assessment methods

The course is closed by a course-unit credit. The students obtain their credits for active attendance at the course and for the elaboration of protocols (4).

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

Bi7430c Molecular biotechnology - practical course

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy)

Guaranteed by

doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.

Prerequisites (in Czech)

NOW( Bi7430 Molecular biotechnology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Microbiology)
• General Biology (programme PřF, N-BI, specialization Microbiology)

Course objectives

Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.

Syllabus

• 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.

Literature

• G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
• F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.

• Enrolment Statistics (recent)