Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2025
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (assistant)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (seminar tutor)
Mgr. Michaela Beránková (assistant)
Mgr. Hana Šimečková (assistant)
Mgr. Adam Vinco (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Ivana Mašlaňová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 18/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
Learning outcomes
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
  • 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course will be held in blocks, in four 3-hour theoretical blocks and in practical blocks, which will be organized twice a week for 3 hours over the course of 4 weeks. Students will design different plasmid constructs and perform practical experiments based on available protocols.
Assessment methods
The prerequisite for completion of the course is writing the initial theoretical test before the pracical part, submitting the protocols and participation in all practical parts of course.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every other week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2024
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (assistant)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (seminar tutor)
Mgr. Michaela Beránková (assistant)
Mgr. Kristína Rovňáková (assistant)
Mgr. Hana Šimečková (assistant)
Mgr. Adam Vinco (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Ivana Mašlaňová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8313/01: Mon 19. 2. to Sun 26. 5. Mon 12:00–14:50 D36/216, L. Kuntová, I. Mašlaňová, R. Pantůček
Bi8313/02: Mon 19. 2. to Sun 26. 5. Tue 13:00–15:50 D36/216, L. Kuntová, I. Mašlaňová, R. Pantůček
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 13/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
Learning outcomes
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
  • 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course will be held in blocks, in four 3-hour theoretical blocks and in practical blocks, which will be organized twice a week for 3 hours over the course of 4 weeks. Students will design different plasmid constructs and perform practical experiments based on available protocols.
Assessment methods
The prerequisite for completion of the course is writing the initial theoretical test before the pracical part, submitting the protocols and participation in all practical parts of course.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (assistant)
Mgr. Michaela Beránková (assistant)
Mgr. Lenka Kosečková Micenková, Ph.D. (assistant)
Mgr. Radka Obořilová (assistant)
Mgr. Hana Šimečková (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 12:00–14:50 D36/216, Tue 12:00–14:50 D36/216, Tue 12:00–14:50 E25/209
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 7/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
Learning outcomes
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
  • 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course will be held in blocks, in four 3-hour theoretical blocks and in practical blocks, which will be organized twice a week for 3 hours over the course of 4 weeks. Students will design different plasmid constructs and perform practical experiments based on available protocols.
Assessment methods
The prerequisite for completion of the course is writing the initial theoretical test before the pracical part, submitting the protocols and participation in all practical parts of course.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2022
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (seminar tutor)
Mgr. Jiří Holoubek, Ph.D. (assistant)
Mgr. Radka Obořilová (assistant)
Mgr. Hana Šimečková (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 12:00–15:50 E25/119, Thu 8:00–8:50 E25/119
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 1/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
Learning outcomes
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
  • 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course will be held in blocks, in one 3-hour theoretical block, which will be common for both seminar groups and in four 6-hour practical blocks for each seminar group separately (max. 12 students per group). Students will design different plasmid constructs and perform practical experiments based on available protocols.
Assessment methods
The prerequisite for completion of the course is writing the initial theoretical test, submitting the protocols and participation in the practical parts of course.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2021
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Hana Šimečková (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 24. 5. 9:00–10:50 E25/119, Tue 25. 5. 9:00–10:50 E25/119, Wed 26. 5. 9:00–10:50 E25/119, Thu 27. 5. 9:00–10:50 E25/119, Fri 28. 5. 9:00–10:50 E25/119, Mon 31. 5. 9:00–10:50 E25/119, Tue 1. 6. 9:00–10:50 E25/119, Wed 2. 6. 9:00–10:50 E25/119, Thu 3. 6. 9:00–10:50 E25/119, Fri 4. 6. 9:00–10:50 E25/119
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
Learning outcomes
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
  • 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2020
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Hana Šimečková (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 8:00–11:50 E25/209
  • Timetable of Seminar Groups:
Bi8313/01: Fri 9:00–12:50 E25/119, I. Mašlaňová, R. Pantůček
Bi8313/02: Wed 8:00–11:50 E25/119, I. Mašlaňová, R. Pantůček
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
Learning outcomes
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
  • 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2019
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8313/01_1: Mon 18. 2. to Fri 17. 5. Wed 9:00–12:50 E25/119, I. Mašlaňová, R. Pantůček
Bi8313/01_2: Mon 18. 2. to Fri 17. 5. Wed 9:00–12:50 E25/119, I. Mašlaňová, R. Pantůček
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
Learning outcomes
At the end of this course, students should be able to perform methods manipulating with recombinant DNA and protein purification.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
  • 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
spring 2018
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8313/01_1: Wed 9:00–12:50 E25/119, P. Bárdy, I. Mašlaňová, R. Pantůček, M. Zeman
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
Learning outcomes
At the end of this course, students should be able to perform methods manipulating with recombinant DNA and protein purification.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
  • 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
  • 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2017
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Wed 9:00–12:50 E25/119, Thu 14:00–15:50 E25/119
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
  • 4. Modification of DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2016
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Mgr. Ivana Kupčíková, DiS. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8313/01_1: Wed 9:00–12:50 E25/119, R. Pantůček
Bi8313/01_2: Wed 9:00–12:50 E25/119, R. Pantůček
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
  • 4. Modification of DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2015
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–9:50 E25/209, Wed 9:00–12:50 E25/119, Fri 9:00–10:50 E25/119
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
  • 4. Modification of DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2014
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 8:00–11:50 E25/119
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
  • 4. Modification of DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2013
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Fri 9:00–9:50 Bpt,01013, Fri 10:00–12:50 Bpt,01013
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2012
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Fri 8:00–8:50 Bpt,01013, Fri 9:00–11:50 Bpt,01013
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h courses in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2011
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Timetable
Fri 8:00–11:50 Bpt,01013
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2010
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2009
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, preparation of molecular probes, use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2008
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh, zápočtového testu a odevzdání protokolů.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2007
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh, zápočtového testu a odevzdání protokolů.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2006
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2005
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2004
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2003
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
  • 4. Modification od DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
Syllabus
  • Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
Literature
Assessment methods (in Czech)
Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh, zápočtového testu a odevzdání protokolů.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)