Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2025
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (assistant)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (seminar tutor)
Mgr. Michaela Beránková (assistant)
Mgr. Hana Šimečková (assistant)
Mgr. Adam Vinco (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Ivana Mašlaňová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 19/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
- Learning outcomes
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
- 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course will be held in blocks, in four 3-hour theoretical blocks and in practical blocks, which will be organized twice a week for 3 hours over the course of 4 weeks. Students will design different plasmid constructs and perform practical experiments based on available protocols.
- Assessment methods
- The prerequisite for completion of the course is writing the initial theoretical test before the pracical part, submitting the protocols and participation in all practical parts of course.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every other week.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2024
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (assistant)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (seminar tutor)
Mgr. Michaela Beránková (assistant)
Mgr. Kristína Rovňáková (assistant)
Mgr. Hana Šimečková (assistant)
Mgr. Adam Vinco (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Ivana Mašlaňová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi8313/01: Mon 19. 2. to Sun 26. 5. Mon 12:00–14:50 D36/216, L. Kuntová, I. Mašlaňová, R. Pantůček
Bi8313/02: Mon 19. 2. to Sun 26. 5. Tue 13:00–15:50 D36/216, L. Kuntová, I. Mašlaňová, R. Pantůček - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 13/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
- Learning outcomes
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
- 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course will be held in blocks, in four 3-hour theoretical blocks and in practical blocks, which will be organized twice a week for 3 hours over the course of 4 weeks. Students will design different plasmid constructs and perform practical experiments based on available protocols.
- Assessment methods
- The prerequisite for completion of the course is writing the initial theoretical test before the pracical part, submitting the protocols and participation in all practical parts of course.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2023
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Ing. Sylva Koudelková, Ph.D. (assistant)
Mgr. Michaela Beránková (assistant)
Mgr. Lenka Kosečková Micenková, Ph.D. (assistant)
Mgr. Radka Obořilová (assistant)
Mgr. Hana Šimečková (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 12:00–14:50 D36/216, Tue 12:00–14:50 D36/216, Tue 12:00–14:50 E25/209
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 7/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
- Learning outcomes
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
- 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course will be held in blocks, in four 3-hour theoretical blocks and in practical blocks, which will be organized twice a week for 3 hours over the course of 4 weeks. Students will design different plasmid constructs and perform practical experiments based on available protocols.
- Assessment methods
- The prerequisite for completion of the course is writing the initial theoretical test before the pracical part, submitting the protocols and participation in all practical parts of course.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2022
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Lucie Kuntová, Ph.D. (seminar tutor)
Mgr. Jiří Holoubek, Ph.D. (assistant)
Mgr. Radka Obořilová (assistant)
Mgr. Hana Šimečková (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 12:00–15:50 E25/119, Thu 8:00–8:50 E25/119
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 1/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9. Proteins purification.
- Learning outcomes
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 2. Expresion and purification of recombinant protein SH3b_GFP by liquid chromatography.
- 3. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 4. Design of plasmid vector using CRISPR-Cas10 system for phage genome editing. The task includes the design of anti-tag sequence, design of a spacer for selection of phage particles. Verification of the functionality of the proposed editing system.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course will be held in blocks, in one 3-hour theoretical block, which will be common for both seminar groups and in four 6-hour practical blocks for each seminar group separately (max. 12 students per group). Students will design different plasmid constructs and perform practical experiments based on available protocols.
- Assessment methods
- The prerequisite for completion of the course is writing the initial theoretical test, submitting the protocols and participation in the practical parts of course.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2021
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Hana Šimečková (seminar tutor) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 24. 5. 9:00–10:50 E25/119, Tue 25. 5. 9:00–10:50 E25/119, Wed 26. 5. 9:00–10:50 E25/119, Thu 27. 5. 9:00–10:50 E25/119, Fri 28. 5. 9:00–10:50 E25/119, Mon 31. 5. 9:00–10:50 E25/119, Tue 1. 6. 9:00–10:50 E25/119, Wed 2. 6. 9:00–10:50 E25/119, Thu 3. 6. 9:00–10:50 E25/119, Fri 4. 6. 9:00–10:50 E25/119
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice || Bi6400c Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
- Learning outcomes
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
- 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2020
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Hana Šimečková (seminar tutor) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 8:00–11:50 E25/209
- Timetable of Seminar Groups:
Bi8313/02: Wed 8:00–11:50 E25/119, I. Mašlaňová, R. Pantůček - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (extraction and purification of DNA, enzymatic reactions, gel electrophoresis) a bioinformatics (manipulating sequence data and pimer design). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
- Learning outcomes
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Cloning of endolysin gene in pUC and pET plasmid vectors (modification of DNA ends by PCR, cloning of PCR products, dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
- 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2019
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi8313/01_1: Mon 18. 2. to Fri 17. 5. Wed 9:00–12:50 E25/119, I. Mašlaňová, R. Pantůček
Bi8313/01_2: Mon 18. 2. to Fri 17. 5. Wed 9:00–12:50 E25/119, I. Mašlaňová, R. Pantůček - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
- Learning outcomes
- At the end of this course, students should be able to perform methods manipulating with recombinant DNA and protein purification.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
- 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of Sciencespring 2018
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi8313/01_1: Wed 9:00–12:50 E25/119, P. Bárdy, I. Mašlaňová, R. Pantůček, M. Zeman
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends, prepare DNA samples for sequencing, edit and silence genes in vivo using CRISPR/Cas9.
- Learning outcomes
- At the end of this course, students should be able to perform methods manipulating with recombinant DNA and protein purification.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Silencing of septum-coding genes using CRISPR/Cas9 in Escherichia coli. Design of guide RNA for CRISPR editing. Manipulation with dCas9 plasmid. Cassette exchange of insert. Transformation of the cells. Evaluation of the results by light microscopy.
- 3. Construction of protein probe for pathogen identification and antimicrobial protein with extended host-range. CRISPR/Cas9 editing of virus particles. Expression and purification of protein using affinity chromatography. Evaluation of the results by multi-spectrometer and chloroform assay.
- 4. Antibiotic resistance gene knock-out in methicillin-resistant Staphylococcus aureus using transposon mutagenesis. Electroporation of foreign DNA. Preparation of competent cells. Mutation screening.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2017
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 20. 2. to Mon 22. 5. Wed 9:00–12:50 E25/119, Thu 14:00–15:50 E25/119
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
- 4. Modification of DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2016
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Mgr. Ivana Kupčíková, DiS. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi8313/01_1: Wed 9:00–12:50 E25/119, R. Pantůček
Bi8313/01_2: Wed 9:00–12:50 E25/119, R. Pantůček - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
- 4. Modification of DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2015
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 9:00–9:50 E25/209, Wed 9:00–12:50 E25/119, Fri 9:00–10:50 E25/119
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
- 4. Modification of DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2014
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 8:00–11:50 E25/119
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
- 4. Modification of DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2013
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Fri 9:00–9:50 Bpt,01013, Fri 10:00–12:50 Bpt,01013
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2012
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Fri 8:00–8:50 Bpt,01013, Fri 9:00–11:50 Bpt,01013
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in six 4 h courses in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2011
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Timetable
- Fri 8:00–11:50 Bpt,01013
- Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2010
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2009
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, preparation of molecular probes, use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2008
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh, zápočtového testu a odevzdání protokolů.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2007
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh, zápočtového testu a odevzdání protokolů.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2006
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2005
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2004
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2003
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering )
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of Sciencespring 2012 - acreditation
The information about the term spring 2012 - acreditation is not made public
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2011 - only for the accreditation
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification od DNA ends and prepare DNA samples for sequencing.
- Syllabus
- 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
- 2. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
- 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection).
- 4. Modification od DNA ends by PCR, cloning of PCR products.
- 5. DNA sequencing.
- Literature
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- The course is taught in one week block in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
Bi8313 Genetic Engineering - Laboratory Course
Faculty of ScienceSpring 2008 - for the purpose of the accreditation
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D. - Prerequisites
- ! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Methods of molecular biology (lecture and practice). - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- Methods of DNA cloning, preparation of molecular probes, polymerase chain reaction and their modifications.
- Syllabus
- Isolation of Sau3AI and Sau96I restriction endonucleases. Cloning in pUC plasmid vectors (defosforylation of DNA ends by alcalic phosphatase, ligaion of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids). DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern bloting, hybridization with labeled probe, immunologic detection). PCR (polymerase chain reaction), RAPD-PCR. DNA sequencing.
- Literature
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Assessment methods (in Czech)
- Výuka probíhá blokově v období květen - červen. Předpokladem pro získání zápočtu je absolvování všech úloh, zápočtového testu a odevzdání protokolů.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
- Enrolment Statistics (recent)