Course Information

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Ing. Matěj Masař (assistant)
Mgr. Sofia Sharkawy (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C15/212, L. Faltinek
C3600/02: Wed 8:00–12:50 C15/212, J. Kučera, M. Masař
C3600/03: Thu 8:00–12:50 C15/212, M. Pelcová
C3600/04: Fri 8:00–12:50 C15/212, L. Faltinek

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• 01. Work safety rules. Organization of laboratory exercises. Preparation of dilute solution, pipetting, and photometry.
• 02. Isolation, analysis, and determination of deoxyribonucleic acid (DNA).
• 03. Qualitative and quantitative determination of amino acids.
• 04. Quantitative determination of proteins.
• 05. Qualitative and quantitative determination of carbohydrates.
• 06. Lipids and their analysis.
• 07. Sucrase - immobilization, substrate specificity, activity determination.
• 08. pH optimum of enzyme reaction.
• 09. Enzyme kinetics.
• 10. Analytical use of enzymes.
• 11. Biological electron transfer chains.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 60% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Bc. Lenka Jurasová (assistant)
Mgr. Martina Rievajová (assistant)
Mgr. Dorota Sklenárová (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C15/212, L. Faltinek, M. Rievajová
C3600/02: Wed 8:00–12:50 C15/212, L. Faltinek, D. Sklenárová
C3600/03: Thu 8:00–12:50 C15/212, L. Faltinek, L. Jurasová
C3600/04: Fri 8:00–12:50 C15/212, L. Faltinek

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Lukáš Faltinek (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jakub Červinka (assistant)
Mgr. Michaela Pešková (assistant)
Mgr. Michal Sedlák (assistant)
Mgr. Sandra Thalerová, Ph.D. (assistant)
Mgr. Tomáš Zlámal (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C15/212, L. Faltinek, M. Sedlák
C3600/02: Wed 8:00–12:50 C15/212, J. Červinka, L. Faltinek
C3600/03: Thu 8:00–12:50 C15/212, L. Faltinek, M. Pešková
C3600/04: Fri 8:00–12:50 C15/212, L. Faltinek, Z. Farka, T. Zlámal

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Lukáš Faltinek (assistant)
Mgr. Tereza Hlaváčová (assistant)
Daniil Kabanov, Ph.D. (assistant)
Ing. Jakub Máčala (assistant)
Mgr. Radka Obořilová (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C15/212, Z. Farka, R. Obořilová
C3600/02: Wed 8:00–12:50 C15/212, L. Faltinek, M. Pelcová
C3600/03: Thu 8:00–12:50 C15/212, K. Dadáková, D. Kabanov
C3600/04: Thu 13:00–17:50 C15/212, J. Máčala, V. Sedláček
C3600/05: Fri 8:00–12:50 C15/212, T. Hlaváčová, J. Kučera

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Veronika Farková, Ph.D. (assistant)
Mgr. Adam Janotík (assistant)
Daniil Kabanov, Ph.D. (assistant)
Mgr. Jakub Sopoušek, Ph.D. (assistant)
Ing. Tatiana Staroňová, Ph.D. (assistant)
Mgr. Šimon Vrana, Ph.D. (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/B1: Mon 17. 5. 8:00–15:00 C15/212, Mon 24. 5. 8:00–15:00 C15/212, K. Dadáková, Z. Farka, J. Sopoušek
C3600/B2: Tue 18. 5. 8:00–15:00 C15/212, Tue 25. 5. 8:00–15:00 C15/212, A. Janotík, J. Kučera
C3600/B3: Wed 19. 5. 8:00–15:00 C15/212, Wed 26. 5. 8:00–15:00 C15/212, D. Kabanov, J. Kučera
C3600/B4: Thu 20. 5. 8:00–15:00 C15/212, Thu 27. 5. 8:00–15:00 C15/212, P. Boublíková, T. Staroňová
C3600/01: Mon 7:00–11:50 C15/212, P. Boublíková, T. Staroňová
C3600/02: Wed 7:00–11:50 C15/212, P. Boublíková, D. Kabanov
C3600/03: Fri 8:00–12:50 C15/212, K. Dadáková, V. Farková
C3600/04: Thu 8:00–12:50 C15/212, P. Boublíková, A. Janotík
C3600/05: Wed 12:00–16:50 C15/212, V. Sedláček
C3600/06: Mon 14:00–18:50 C15/212, Z. Farka, J. Sopoušek
C3600/07: Fri 14:00–18:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C15/212, P. Boublíková
C3600/02: Wed 8:00–12:50 C15/212, P. Boublíková
C3600/03: Fri 8:00–12:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 17. 9. to Fri 14. 12. Mon 8:00–12:50 C15/212, P. Boublíková
C3600/02: Mon 17. 9. to Fri 14. 12. Wed 8:00–12:50 C15/212, P. Boublíková
C3600/03: Mon 17. 9. to Fri 14. 12. Fri 8:00–12:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 18. 9. to Fri 15. 12. Mon 8:00–12:50 C15/212, P. Boublíková
C3600/02: Mon 18. 9. to Fri 15. 12. Wed 8:00–12:50 C15/212, P. Boublíková
C3600/03: Mon 18. 9. to Fri 15. 12. Fri 8:00–12:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 19. 9. to Sun 18. 12. Mon 8:00–12:50 C15/212, P. Boublíková
C3600/02: Mon 19. 9. to Sun 18. 12. Wed 8:00–12:50 C15/212, P. Boublíková
C3600/03: Mon 19. 9. to Sun 18. 12. Fri 8:00–12:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Bc. Dana Peňázová (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 8:00–12:50 C15/212, P. Boublíková
C3600/02: Wed 8:00–12:50 C15/212, P. Boublíková
C3600/03: Fri 8:00–12:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Bc. Dana Peňázová (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Wed 8:00–12:50 C15/212, P. Boublíková
C3600/03: Mon 8:00–12:50 C15/212, P. Boublíková
C3600/04: Fri 9:00–13:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Bc. Dana Peňázová (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Wed 8:00–12:50 C15/211, P. Boublíková
C3600/02: Fri 8:00–12:50 C15/211, P. Boublíková
C3600/03: Thu 8:00–12:50 C15/211, P. Boublíková
C3600/04: Mon 8:00–12:50 C15/211, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Wed 9:00–13:50 C15/212, P. Boublíková
C3600/02: Thu 8:00–12:50 C15/212, P. Boublíková
C3600/03: Mon 9:00–13:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Wed 9:00–13:50 C15/212, P. Boublíková
C3600/02: Thu 8:00–12:50 C15/212, P. Boublíková
C3600/03: Mon 9:00–13:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Wed 8:00–12:50 C15/212, P. Boublíková
C3600/02: Thu 8:00–12:50 C15/212, P. Boublíková
C3600/03: Fri 8:00–12:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C05/108, P. Boublíková
C3600/02: Tue 14:00–18:50 C05/108, P. Boublíková
C3600/03: Wed 12:00–16:50 C05/108, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 8:00–12:50 C05/108, P. Boublíková
C3600/02: Tue 7:00–11:50 C05/108, P. Boublíková
C3600/03: Wed 15:00–19:50 C05/108, P. Boublíková
C3600/04: Fri 11:00–15:50 C05/108, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C05/113, P. Boublíková
C3600/02: Wed 14:00–18:50 C05/113, P. Boublíková
C3600/03: Fri 7:00–11:50 C05/113, P. Boublíková
C3600/04: Fri 12:00–16:50 C05/113, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Heat dependence of enzyme reaction. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

RNDr. Pavla Boublíková, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Tue 15:00–19:50 C02/113, P. Boublíková
C3600/02: Fri 12:00–16:50 C02/113, P. Boublíková
C3600/03: Thu 15:00–19:50 C02/113, P. Bouchal
C3600/04: Fri 7:00–11:50 C02/113, J. Lochman

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Heat dependence of enzyme reaction. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)

Guaranteed by

RNDr. Pavla Boublíková, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Fri 7:00–11:50 C02/113, P. Boublíková
C3600/02: Thu 14:00–18:50 C02/113, P. Boublíková
C3600/03: Fri 12:00–16:50 C02/113, P. Bouchal

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Heat dependence of enzyme reaction. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

RNDr. Pavla Boublíková, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Fri 10:00–14:50 Cpl,02022, P. Boublíková
C3600/02: Thu 14:00–18:50 Cpl,02022, M. Mandl

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.

Syllabus

• Qualitative reactions of carbohydrates. Determination of glucose using a biosensor. Carbohydrate thin-layer chromatography.
• Isolation and identification of lipids from the egg yolk.
• Protein electrophoresis of the blood serum. Determination and chromatographic separation of leaf pigments. Gel permeation chromatography in separation of biomolecules.
• Chemical reactions of amino acids and proteins. Determination of amino acids and peptides. Paper chromatography of amino acids. Protein isolation.
• Isolation and properties of nucleic acids.
• Heat dependence of enzyme reaction.
• Protein determination.
• pH effect on enzyme reaction. Determination of the rate of enzyme reaction.
• Substrate specifity of enzymes. Determination of enzyme activity.
• Enzyme kinetics. Enzyme inhibition.
• Enzymatic determination of metabolites.
• Electron transport chain of aerobic organisms.
• Closing test.

Literature

• MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: No timetable has been entered into IS. P. Skládal
C3600/02: No timetable has been entered into IS. M. Mandl
C3600/03: No timetable has been entered into IS. P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.

Syllabus

• Qualitative reactions of carbohydrates. Determination of glucose using a biosensor. Carbohydrate thin-layer chromatography.
• Isolation and identification of lipids from the egg yolk.
• Protein electrophoresis of the blood serum. Determination and chromatographic separation of leaf pigments. Gel permeation chromatography in separation of biomolecules.
• Chemical reactions of amino acids and proteins. Determination of amino acids and peptides. Paper chromatography of amino acids. Protein isolation.
• Isolation and properties of nucleic acids.
• Heat dependence of enzyme reaction.
• Protein determination.
• pH effect on enzyme reaction. Determination of the rate of enzyme reaction.
• Substrate specifity of enzymes. Determination of enzyme activity.
• Enzyme kinetics. Enzyme inhibition.
• Enzymatic determination of metabolites.
• Electron transport chain of aerobic organisms.
• Closing test.

Literature

• MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/Cb29: No timetable has been entered into IS. P. Boublíková
C3600/Cb28: No timetable has been entered into IS. M. Mandl
C3600/Cb27: No timetable has been entered into IS. P. Skládal

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.

Syllabus

• Qualitative reactions of carbohydrates. Determination of glucose using a biosensor. Carbohydrate thin-layer chromatography.
• Isolation and identification of lipids from the egg yolk.
• Protein electrophoresis of the blood serum. Determination and chromatographic separation of leaf pigments. Gel permeation chromatography in separation of biomolecules.
• Chemical reactions of amino acids and proteins. Determination of amino acids and peptides. Paper chromatography of amino acids. Protein isolation.
• Isolation and properties of nucleic acids.
• Heat dependence of enzyme reaction.
• Protein determination.
• pH effect on enzyme reaction. Determination of the rate of enzyme reaction.
• Substrate specifity of enzymes. Determination of enzyme activity.
• Enzyme kinetics. Enzyme inhibition.
• Enzymatic determination of metabolites.
• Electron transport chain of aerobic organisms.
• Closing test.

Literature

• MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
Mgr. Michal Kuňák, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Ecotoxicology (programme PřF, N-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

RNDr. Pavla Boublíková, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, B-BI)
• Molecular Biology and Genetics (programme PřF, M-BI)
• Molecular Biology and Genetics (programme PřF, N-BI)

Course objectives

The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Heat dependence of enzyme reaction. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

• Enrolment Statistics (recent)