Bi5220c Immunology - practical course

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Alena Žákovská, Ph.D. (seminar tutor)
MVDr. Mgr. Monika Dušková, Ph.D. (seminar tutor)
Mgr. Karel Vostal (seminar tutor)
Guaranteed by
doc. RNDr. Alena Žákovská, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Žákovská, Ph.D.
Prerequisites (in Czech)
Bi5220 Immunology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: understand and explain practical usage of basic immunological techniques; work with information on practical usage of basic immunological techniques; create ... ; make reasoned decisions about basic immunological techniques; make deductions based on acquired knowledge of basic immunological techniques; interpret methodology used in immunology research;
Syllabus
  • 1. Histology of immune organs 2. Cells involved in the immune system 3. Determination of anti-microbial activity: lysosyme by method of radial diffusion 4. Phagocyte activity: test of ingestion of MSH particles 5. Phagocyte activity: chemiluminiscent determination 6. Lymphocyte isolation: method of gradient sedimentation 7. Chemiluminiscent method in evaluation od complement activity 8. Determination of antibodies: immuno-diffusion and turbidimetry 9. Proteins in blood serum: immunophoresis 11. Hemocytes: determination of anti-microbial activity
Literature
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. Vyd. 1. V Praze: Triton, 1998, 219 s. ISBN 808587573X. info
  • PROCHÁZKOVÁ, Jitka and Ctirad JOHN. Vybrané diagnostické metody lékařské imunologie. 1. vyd. Praha: Avicenum - zdravotnické nakladatelství, 1986, 367 s. URL info
Teaching methods
practical course
Assessment methods
Exercises are compulsory,protocols, final exam – in written form
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.