Bi9042 Analysis of chromatin structure - practical training

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Jiří Fajkus, CSc. (seminar tutor)
Mgr. Eva Sýkorová, CSc. (seminar tutor)
doc. Mgr. Petra Procházková Schrumpfová, Ph.D. (seminar tutor)
doc. Mgr. Miloslava Fojtová, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi9041 Struct. euk. chromosomes )
Basic knowledge of molecular biology and biochemistry, parallel or previous subscription of the course Structure and function of eukaryotic chromosomes (Bi9041), knowledge of laboratory safety instructions
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
In the end of the course, students will be practically able to apply knowledge gained in the lecture course Structure and function of eukaryotic chromosomes (Bi9041). The aim of the course is to provide students with practical experience with methods used in analysis of the structure of chromosomes and chromatin. Main objectives: Student will be able: to isolate cell nuclei
to learn how to isolate and analyse histones
to learn MNase digestion of chromatin and analyse nucleosome spacing
Syllabus
  • 1. Isolation of cell nuclei from animal and plant tissues 2. Digestion of nuclei with micrococcal nuclease 3. Extraction of nucleosomal DNA 3. Analysis of nucleosomal DNA by electrophoresis 4. Preparation of mononucleosomal DNA from agarose gel 5. Mapping of nucleosome position using restriction enzymes or primer extension 6. Computer modelling of nucleosome position 7. Comparison of experimental and computer-predicted results 8. Extraction of chromatin proteins 9. Analysis of chromatin proteins by SDS-PAGE
Literature
  • FAJKUS, Jiří, Andrew R. LEITCH, Michael CHESTER and Eva SÝKOROVÁ. Evolution, Composition and Interrelated Functions of Telomeres and Subtelomeres: Lessons from Plants. In Origin and Evolution of Telomeres. USA: Landes Biosciences, 2008, p. 114-127. ISBN 978-1-58706-309-1. info
  • FAJKUS, Jiří and Edward N. TRIFONOV. Columnar Packing of Telomeric Nucleosomes. Biochemical and Biophysical Research Communications. California: Academic Press, 2001, roč. 280, No 4, p. 961-963. ISSN 0006-291X. info
Teaching methods
Practical laboratory training - intensive block teaching
Assessment methods
Written protocol emphasizing problems met during the project solving and discussion of the results. Final group discussion of the course experience.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.