PřF:Bi4035 Molecular biology - lab.pract. - Course Information
Bi4035 Molecular biology - laboratory practice
Faculty of ScienceSpring 2012
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. Mgr. Petr Beneš, Ph.D. (seminar tutor)
Mgr. Andrea Fořtová, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi4035/01: Wed 13:00–16:50 BprR, V. Růžičková
Bi4035/02: Thu 13:00–16:50 BprR, V. Růžičková
Bi4035/03: Wed 14:00–17:50 C13/332, P. Beneš - Prerequisites
- ( C1080 General Chemistry-lab.course || C1620 General and Inorg.Chem.-lab. || C1650 Fundamentals of Chem.-lab. || C2100 Inorganic Chemistry-lab.course || C2500 General Chemistry-lab.course || C2740 Fundament. of Chem.-lab.course || C2480 Basic Organic Chem. and Bioch. || SOUHLAS) && ( Bi4020 Molecular biology || NOW( Bi4020 Molecular biology )) && ! B4020 Molecular biology && ! B7940 Molecular biology && ! B3120 Molecular and cell biology && ! Bi4020c Essential methods in mol. biol
Znalost molekulární struktury a funkce prokaryotického a eukaryotického genomu. Znalost základních mikrobiologických technik jako kultivace bakterií, stanovení titru bakteriální suspenze. Znalost výpočtů složení chemických roztoků a jejich příprava. Předpokládá se předchozí absolvování základních cvičení z anorganické a organické chemie a mikrobiologie. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 135 student(s).
Current registration and enrolment status: enrolled: 0/135, only registered: 0/135 - fields of study / plans the course is directly associated with
- there are 13 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to competently analyse the genetic material including chromosome DNA and solve experiments with plasmids originating from the bacteria.
- Syllabus
- This course include the practical tasks which serve to acquire the basic methods in the molecular biology, primarily, isolation and manipulation with nucleic acids.Preparation, solutions usefull for molecular methods: Isolation of plasmid DNA Concentration and purity of DNA Restriction splitting, ELFO Ultracentrifugation
- Literature
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- Study materials are available in the Is.
- Assessment methods
- Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi4020c Molecular biology - laboratory practice
(C1600 || C1080 || C1620 || C1650 || C2100 || C2500 || C2740 || C2480 || Bi9410 || souhlas) && (Bi4020 || NOW(Bi4020)) && !NOWANY(Bi4010c,Bi4035) && !Bi4010c && !Bi4035 - Bi6400c Methods of Molecular Biology - practice
(Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020 || Bi4035) && NOW(Bi6400) && !B6405
- Bi4020c Molecular biology - laboratory practice
- Enrolment Statistics (Spring 2012, recent)
- Permalink: https://is.muni.cz/course/sci/spring2012/Bi4035