PřF:C4220 Biochemistry-lab.course - Course Information
C4220 Biochemistry - laboratory course
Faculty of ScienceSpring 2023
- Extent and Intensity
- 0/0/7. 7 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. Lukáš Faltinek (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Taťána Bržezická (assistant)
Mgr. Šárka Kobzová (assistant)
Mgr. Eliška Macháčová (assistant)
Mgr. Michaela Pešková (assistant) - Guaranteed by
- doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable of Seminar Groups
- C4220/01: Mon 8:00–14:50 C15/212, L. Faltinek, Š. Kobzová
C4220/02: Wed 8:00–14:50 C15/212, L. Faltinek, M. Pešková
C4220/03: Thu 8:00–14:50 C15/212, T. Bržezická, M. Pelcová
C4220/04: Fri 8:00–14:50 C15/212, K. Dadáková, E. Macháčová
C4220/05: Tue 12:00–18:50 C15/212, J. Kučera - Prerequisites
- C3181 Biochemistry I && ( C4221 Introductory Biochemistry Laboratory || C1100 Introductory Chemistry Lab )
Basic knowledge of Biochemistry I - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Chemistry (programme PřF, N-CH)
- Inorganic Chemistry (programme PřF, N-CH)
- Applied Biochemistry (programme PřF, B-AB)
- Biochemistry (programme PřF, B-BCH)
- Biochemistry (programme PřF, N-CH)
- Physical Chemistry (programme PřF, N-CH)
- Chemistry for Multi-Branches Study (programme PřF, B-CH)
- Chemistry (programme PřF, B-CH)
- Environmental Chemistry (programme PřF, N-CH)
- Macromolecular Chemistry (programme PřF, N-CH)
- Organic Chemistry (programme PřF, N-CH)
- Course objectives
- At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of metabolic pathways. Physiological processes and their biochemical nature. Basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes.
- Learning outcomes
- Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work
- Syllabus
- Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.
- Literature
- Teaching methods
- laboratory courses
- Assessment methods
- The first exercise is devoted to learning about safety in biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Information on course enrolment limitations: V podzimním semestru se vypisuje pouze jedna skupina pro 12 studentů chemických oborů jako náhrada za JS
- Enrolment Statistics (Spring 2023, recent)
- Permalink: https://is.muni.cz/course/sci/spring2023/C4220