C8302 Proteomics - practice

Faculty of Science
Spring 2023
Extent and Intensity
0/0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Danuše Fridrichová (seminar tutor)
Ing. Eliška Kadlecová (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)
Mgr. Kateřina Konečná (assistant)
Guaranteed by
doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites (in Czech)
CG090 Proteomic methods ||NOW( CG090 Proteomic methods )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 2/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
The goal of the course is deepening the theoretical knowledge and getting hands-on experience in the fields of proteome analysis using mass spectrometry, protein separation, preparation of recombinant DNA, expression and purification of recombinant proteins and their functional characterization.
Learning outcomes
After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western blotting
  • Work with protein databases
  • Determining 3D conformation of proteins with similar AA sequence
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Teacher's information
All the tasks of the practical course will be taught in a week-long block, if not announced differently, then always in the last week of a given semester.
The course is also listed under the following terms Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/C8302