B5780 Zoological microtechnique

Faculty of Science
Autumn 2001
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: graded credit.
Teacher(s)
RNDr. Božena Koubková, Ph.D. (lecturer)
Guaranteed by
RNDr. Božena Koubková, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: RNDr. Božena Koubková, Ph.D.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Basic aspect of light microscopy with a view to the zoological methods - dark field, phase contrast, differential interference contrast, fluorescence microscopy,measurement methods, drawing, photomicrography). Basic zoological techniques - native and permanent slides, fixation, staining, mounting media, embedding and cutting.
Syllabus
  • 1. Basic aspects of light microscopy, types of microscopy,the compound microscope and Köhler illumination,lens nomenclature 2. Measurements methods, calibration, ocular micrometer, stage micrometer, digital analysis, drawing 3. Contrast methods, dark-field, phase-contrast, differential interference contrast (DIC) 4. Photomicrography, digital photomicrography 5. Native slides, vital staining, Neutral red, Congo red, Methylen green 6. Fixation,properties of some common chemical fixatives 7. Aqueous mounting media, glycerol,glycerol-gelatine, Liquido-Faure, PVA 8. Canada balsam, dehydration, clearing 9. Stains and staining,hematoxylin staining, carmine staining 10.Histological methods, clearing, infiltration, embedding for paraplast method 11.Sectioning, mounting and staining 12.Fluorescence microscopy, fluorescence
Literature
  • Úvod do světelné mikroskopie. Edited by Milan Hejtmánek. 3. přeprac. a dopl. vyd. Olomouc: Vydavatelství University Palackého, 1993, 65 s. ISBN 8070673087. info
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
  • Lacey A.J., Ed.,1989: Light Microscopy in Biology. A Practical Approach. IRL Press at Oxford University Press, 329 pp.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 1999, Autumn 2000.
  • Enrolment Statistics (recent)
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