Bi7311 Molecular Biology of Procaryotes - Laboratory Course

Faculty of Science
Autumn 2008
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites
( B2050 General microbiology || Bi2050c General microbiology pr. || B4070 General mikrobiology || B4090 General microbiology || Bi4090c General microbiology - pr. ) && NOW( Bi7120 Molecular biology of prokaryotes )
Basic knowledge from molecular biology of the Prokaryotes. Basic techniques of microbiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Practical course focused on molecular methods for analysis of procaryotic genome and basic methods of bacterial genetics. At the end of this course, students should be able to design and perfotm basic laboratory experiments focused on genetic studies of Escherichia coli and selected species of Gramm-positive cocci.
Syllabus
  • 1. Pulsed-field gel electrophoresis of bacterial genomic DNA (preparation of samples, digestion with restriction endonuclease SmaI, PFGE).
  • 2. Isolation of spontaneous mutants of Staphylococcus aureus resistant to antibiotics on gradient agar plates.
  • 3. Fluctuation test and estimation of mutation rate.
  • 4. Conjugation of Escherichia coli.
  • 5. Isolation of auxotroph mutants of Staphylococcus aureus. Replica plating.
  • 6. Typing techniques used for epidemiological, and phylogenetical purposes, AP-PCR, RFLP.
Literature
  • MILLER, Jeffrey H. A Short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria. New York: Cold Spring Harbor Laboratory Press, 1992, 456 s. ISBN 0879693495. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods
The course is taught in one week block. The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/Bi7311