Bi9080 Technical processes in biotechnology

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 14:00–15:50 Bpt,01013
Prerequisites (in Czech)
Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Material balances. Fluids properties. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Membrane separation processes. Large scale chromatographic purification. Drying. Storage and stability of proteins. Main objectives can be summarized as follows: understand to the principes of methods and processes used at the isolation of biotechnological products
Syllabus
  • Stechiometry of microbial growth. Material balances.
  • Fluids properties. Fluid transfer in piping.
  • Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
  • Influence of solvents on enzyme activities.
  • Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
  • Precipitation. Salting out. Denaturation and renaturation of proteins.
  • Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
  • . Membrane separation processes. Large scale chromatographic purification of proteins.
  • Drying. Storage and stability of proteins. Validation of production quality.
Literature
  • 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
Assessment methods
Oral exam.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.
  • Enrolment Statistics (Autumn 2008, recent)
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