Bi9901 Free radicals in animal physiology

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Číž, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Antonín Lojek, CSc. (lecturer)
Guaranteed by
doc. RNDr. Milan Číž, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Antonín Lojek, CSc.
Timetable
Tue 15:00–16:50 BFU
Prerequisites (in Czech)
Bi5220 Immunology || Bi6930 Immunotoxicology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
The lecture brings a basic information from the field of molecular principles of origin and activity of reactive oxygen and nitrogen species and their physiological role in animal organism. Reactive oxygen and nitrogen species are further discussed from the point of view of occurrence and development of diseases initiated by oxidative stress.
Syllabus
  • Biology of oxygen (Oxygen and evolution of life on Earth; Oxygen cycle in nature; Photosynthesis; Respiratory chain). Chemistry of free radicals (Reactive oxygen species; Role of transition metal ions; Nitric oxide and derived molecules). Sources of free radicals (Endogenous physiological or pathological processes: Leak of electrons from metabolic pathways, Peroxisomes, Xanthine/xanthine oxidase, Metabolism of arachidonic acid, Autooxidation of catechols and flavonoids; Exogenous sources, Food constituents, Drugs, UV irradiation, Ionising irradiation, Polluted environment). Physiology of free radicals (Phagocytosis; NADPH oxidase of professional phagocytes; Microbicidal mechanisms based on oxygen; Chronic granulomatous disease (CGD); Gene regulation; Apoptosis; Oxidative burst in plants as defence mechanism against infection). Role of free radicals in diseases (Lipid peroxidation; Protein degradation; DNA damage; Regulation of oxidative stress). Defence against free radicals (Prevention of free radical generation; Compartmentalisation of free radicals; Free radical scavengers; Antioxidant defence and damage repair; Antioxidant pharmacological intervention). Pathology of free radicals (Oxidative stress and aging; Reproduction; Ischemia-reperfusion injury; Inflammation; Atherosclerosis; Brain dysfunction; Cancer; Myocarditis; Diabetes mellitus; Eye diseases; Adult respiratory distress syndrome (ARDS); Oxidative injury to lungs induced by xenobiotics; Radiation injury; Rheumatoid arthritis; Skin injury). Methods of oxidative damage evaluation (Detection of free radicals; Evaluation of antioxidant activity of biological samples; Determination of damage to biological macromolecules).
Literature
  • ŠÍPEK, Stanislav. Antioxidanty a volné radikály ve zdraví a v nemoci. 1. vyd. Praha: Grada, 2000, 314 s. ISBN 8071697044. info
Assessment methods
lectures, reading, class discussion, written examination
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught once in two years.
Information on the per-term frequency of the course: každý sudý kalendářní rok.
Information on course enrolment limitations: min. počet zapsatelných studentů: 5
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2016, Autumn 2018, Autumn 2020, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/Bi9901