PřF:Bi9903 Developmental Anim Physiol I - Course Information

Bi9903 Developmental Animal Physiology I

Extent and Intensity

2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).

Teacher(s)

prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
RNDr. Pavel Krejčí, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Mgr. Vítězslav Bryja, Ph.D.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

Main objectives of the course are: - to learn about basic methods and models used in the animal developmental biology - to learn about molecular and cellular mechanisms regulating animal development - to discuss practical aspects associated with the studies of development

Syllabus

• 1. Introduction to developmental biology. General attributes of development: genomic equivalence, cloning, early development, morphogenetic processes. Developmental biology: Developmental mutants and their screening, gene cloning, transgenesis, gene duplication. 2. Experimental embryology. Techniques used in developmental biology: microscopy, methods for gene expression analysis, reporters, microinjections, labeling methods, cell sorting. Main model organisms: model advantages, availability and price, micromanipulation, gene and genome maps. Characterization of 6 main models: Xenopus, Zebrafish, Chicken, Mouse, Drosophila melanogaster, Caenorhabdis elegans. 3. Mouse as a model for developmental biology. Current possibilities of gene manipulation in mouse, accessible systems, advantages, disadvantages and hands-on approaches. 4. Growth, regeneration and evolution. Growth and aging: size and proportion of the body, growth of the skeleton; postnatal growth and differentiation. Growth, aging and death. Molecular mechanisms of aging. Regeneration of the missing body-parts: introduction, regeneration of planarian body, limb regeneration in vertebrates. Ontogenesis and phylogenesis: macroevolution, primary animal. 5. Mechanisms regulating the beginning of development. Oocyte- and sperm- development in mammals, molecular mechanisms ensuring correct localization of maternal mRNA and oocyte-function, molecular mechanisms connected with fertilization, cell processes linked to fertility defects. 6. Key molecular components regulating development I. Molecular characterization of main morphogenetic systems: TGF/BMP, tyrosin kinase receptor (insulin receptor, FGF, ephrins and other tyrosin kinases). Examples of regulated processes. 7. Key molecular components regulating development II. Hedgehog, WNT, Notch, HIF. 8. Key molecular components of development III. Extracellular matrix (interactions with ligands), mechanisms of adhesion and cell migration. Key transcription factors (bHLH, Pax, homeobox proteins etc.), iPS. 9. Mechanisms of development I (FGF) Characterization of the FGF morphogenetic system: Major components, molecular mechanisms of signal transduction, molecular, cellular and tissue phenotypes, the most important mutants, FGF interaction with other morphogens in the limb formation. 10. Mechanisms of development II (Wnt). Major components, molecular mechanisms of signal transduction, molecular, cellular and tissue phenotypes, the most important mutants, Wnt interaction with other morphogens in the development of a selected organ. 11. Marcela Buchtová – tooth and face development. Lecture of external specialist. Aplication of known mechanisms on specific developmental process. 12. Frontiers of developmental biology: Dr. Sigmar Stricker, Max Planck Institute for Molecular Genetics, Berlin, Germany: “Molecular mechanisms and alterations in the limb development”.

Literature

• GILBERT, Scott F. Developmental biology. Edited by Susan R. Singer. 8th ed. Sunderland, Mass.: Sinauer Associates, 2006, xviii, 817. ISBN 087893250X. info
• SLACK, J. M. W. Essential developmental biology. 2nd ed. Oxford: Blackwell, 2006, ix, 365. ISBN 1405122161. info
• WILT, Fred H. and Sarah HAKE. Principles of developmental biology. New York, N.Y.: W.W. Norton & Company, 2004, xxiii, 430. ISBN 0393974308. info
• BROWDER, Leon W., Carol A. ERICKSON and William R. JEFFERY. Developmental biology. 3rd ed. Philadelphia: Saunders College Publishing, 1991, 754 s. ISBN 0-03-013514-1. info

Teaching methods

Teaching methods: lectures, class discussion, group projects, presentations by professionals in the sector

Assessment methods

Colloquium requirements: active participation on the course, presentation of the student project

Language of instruction

Czech

Follow-Up Courses

• Bi9906 Outbound Development Biology Seminar

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Předmět je primárně určen pro studenty magisterských a doktorských studijních programů.

Listed among pre-requisites of other courses

• Bi9903en Developmental Biology Of Animals
  (!Bi9903) && !NOWANY(Bi9903)  

• Enrolment Statistics (Autumn 2011, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2011/Bi9903