C3620 Biochemistry - laboratory course

Faculty of Science
Autumn 2022
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Sandra Thalerová, Ph.D. (assistant)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Thu 14:00–16:50 C15/212
Prerequisites (in Czech)
NOW( C3580 Biochemistry )
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.
Learning outcomes
Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work
Syllabus
  • Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.
Literature
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Teaching methods
laboratory courses
Assessment methods
The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/C3620