E0311 Chromatographic Methods

Přírodovědecká fakulta
podzim 2023
Rozsah
2/0/0. 2 kr. (plus ukončení). Ukončení: zk.
Vyučující
doc. RNDr. Zdeněk Šimek, CSc. (přednášející)
Garance
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Přírodovědecká fakulta
Kontaktní osoba: doc. RNDr. Zdeněk Šimek, CSc.
Dodavatelské pracoviště: RECETOX – Přírodovědecká fakulta
Rozvrh
Po 11:00–12:50 D29/252-RCX1
Předpoklady
Introductory course on Analytical Chemistry or an equivalent
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
předmět má 7 mateřských oborů, zobrazit
Cíle předmětu
At the end of the course, students should be able to:
- define fundamentals of chromatographic methods and describe them in the context with other separation analytical methods;
- understand and clarify principles of individual types of chromatography and functional layout of chromatographic instrumentation, their individual parts and detecting elements;
- explain theoretical aspects and mechanisms of chromatographic separation and principles of choice of chromatographic systems;
- asses and interpret results of chromatographic analyses;
- evaluate significance of connection of chromatographic methods with various analytical techniques, especially with spectral methods;
- take advantage of chromatography in quantitative and qualitative analyses of various types of samples;
Osnova
  • 1. Chromatographic separation, retention parameters, chromatogram, measure of separation, separation efficiency. chromatographic zones.van Deemter equation a Golay equation. Retention indexes.
  • 2. Gas chromatography.
  • 3. Gas-liquid chromatography. Columns, liquid phases, carriers.
  • 4. Adsorption gas chromatography. Characteristics , comparison with GLC. Adsorbents, application.
  • 5. Capilary column, WCOT, PLOT, SCOT. Quality evaluation.
  • 6. Mobile phases, gas mobile phases comparison.
  • 7. GC instrumentation, sample injection, detection.
  • 8. Liquid chromatography. Stationary and mobile phases, separation properties
  • 9. Column, stationary phases, sorbents properties.
  • 10. Mobile phases, classification of solvents, elution techniques.
  • 11. Techniques, principles, retention models, separation strategy, application.
  • 12. Instrumentation, pumps, injection devices, detectors and detection principles.
  • 13. Trends in development of chromatographic methods.
  • 14. Examples of chromatographic applications.
Literatura
  • POOLE, C. F. The essence of chromatography. 1st ed. Amsterdam: Elsevier, 2003, ix, 925. ISBN 0444501991. info
  • POOLE, C. F. a S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • MEYER, Veronika R. Practical High-Performance Liquid Chromatography. 3. vyd. Chichester: J. Wiley & Sons, 1999, 338 s. ISBN 0-471-98372-1. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004, xl, 518. ISBN 0444511067. info
Výukové metody
Teaching is conducted in the form of self-study of study materials in English and individual consultations. Students will receive copies of individual images in PowerPoint and an electronic version stored in IS. The intelligibility of difficult parts is verified interactively during individual consultations.
Metody hodnocení
The final assessment (at the end of the semester) is by oral examination. Three related topics are the content of the discussion during the oral examination.
Vyučovací jazyk
Angličtina
Navazující předměty
Informace učitele
Doc. RNDr. Zdeněk Šimek, CSc.; RECETOX, Faculty of Science, Masaryk University; Kamenice 753/5; 625 00 Brno; Czech Republic; Phone: +420 549 494 964; E-mail: zdenek.simek@recetox.muni.cz; http://www.recetox.muni.cz/
Další komentáře
Studijní materiály
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každý semestr.
Předmět je zařazen také v obdobích podzim 2022, podzim 2024.