C3580 Biochemistry

Faculty of Science
Autumn 2024
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Bouchal, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Bouchal, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 12:00–13:50 B22/116 aula, Tue 14:00–14:50 B11/132
Prerequisites
Basic knowledge from general, physical and organic chemistry and general biology is expected. Attending relevant basic courses is recommended.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The objective of the basic biochemistry lecture (3hrs/week) is to provide the students of (mostly) biological disciplines with a knowledge of biochemistry for their subsequent education and practice, to understand properties and function of biomolecules (nucleic acids, proteins, carbohydrates and lipids), basics of enzymology and molecular physiology, metabolism and biosynthesis of physiologically important biomolecules, mechanisms of ther regulation, as well as relevant laboratory methods.
Learning outcomes
At the end of this course the students should recognize and classify the most important biomolecules and understand their function, will be able to understand biochemical processes (metabolism, biosynthesis) in living systems, mechanisms of enzyme catalysis and their regulation. The student will also understand the key methods of analysis of biomolecules, such as desintegration methods, methods of isolation and purification of proteins, nucleic acids and other biopolymers, chromatographic and electrophoretic methods, spectroscopic and physical methods. The students should be able to apply their knowledge in their scientific practice within bachelor, master and PhD theses and during their scientific career.
Syllabus
  • 1. Introduction
  • 2. Nucleic acids - structure, properties and function, methods of isolation and analysis
  • 3. Aminoacids, peptides and proteins - structure, properties and function, methods of isolation and analysis
  • 4. Carbohydrates - structure, properties and function
  • 5. Lipids - structure, properties and function
  • 6. Enzymology
  • 7. Carbohydrate metabolism - glycolysis, gluconeogenesis, pentose phosphate pathway
  • 8. Citrate cycle, electron transport and oxidative phosphorylation
  • 9. Photosynthesis
  • 10. Lipid metabolism -beta-oxidation and biosynthesis of fatty acids
  • 11. Protein metabolism - deamination, urea cycle, nitrogen metabolism
  • 12. Regulations of biochemical processes
  • 13. Gene expression regulation
Literature
    recommended literature
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry : life at the molecular level. Fifth edition. Hoboken: Wiley, 2016, xviii, 109. ISBN 9781118918401. info
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • Bouchal, Pavel: Prezentace k přednášce C3580 Biochemie
    not specified
  • MURRAY, Robert K. Harperova biochemie. Vyd. v ČR 4., V H & H 3. Jinočany: H & H, 2002, ix, 872. ISBN 8073190133. info
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
Teaching methods
Lectures with a demontration of the taught topic by means of demonstration videos and programs.
Assessment methods
The final exam is in the form of written test, which contains three questions, one from the static biochemistry, one from enzymology and one from metabolism and bioenergetics. The test also contains four chemical formulas of basic biochemical compounds, student should recognize them and describe their biochemical role. The exam also contains a test part with proposed questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
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