VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2000
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
MUDr. Ivo Nentwich, Ph.D. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jindřich Lokaj, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Clinical immunology laboratory
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
  • Laboratory markers of inflammation.
  • Blood collection.
  • Diagnostic antisera.
  • Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum.
  • International units.
  • Direct and indirect agglutination.
  • Blood groups. Rheumatoid factor.
  • Precipitation. Double immune diffusion.
  • Radial immunodiffusion.
  • Turbidimetry, nephelometry.
  • Immunoelectrophoresis, immunofixation.
  • Blotting techniques.
  • Clinical significance of paraproteinemia.
  • Monoclonal antibodies.
  • Immunofluorescence tests.
  • Enzyme analysis (ELISA). RIA.
  • IgE determination.
  • Clinically significant organ specific and organ non-specific autoantibodies.
  • CD nomenclature of leukocyte antigens.
  • Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
  • Evaluation of phagocytic cells.
  • Immunophenotyping of leukemias.
  • Complement system tests.
  • Circulating and localised immune complexes.
  • HLA typing. Molecular genetic in immunology.
  • Skin tests for early hypersensitivity.
  • Delayed-type hypersensitivity tests. Anergy.
  • Prevention and treatment of allergic diseases.
  • Drug hypersensitivity.
  • Anaphylactic shock.
  • Indication and interpretation of immunological tests.
Literature
  • LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info
Assessment methods (in Czech)
Zápočet po absolvování povinné výuky
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (Autumn 2000, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2000/VLIM051c