LF:VLIM051c immunology - practicals

VLIM051c Immunology-practicals

Faculty of Medicine
Autumn 2002

Extent and Intensity

0/2/0. 1 credit(s). Type of Completion: z (credit).

Teacher(s)

Guaranteed by

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

Clinical immunology laboratory
Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins.
Laboratory markers of inflammation.
Blood collection.
Diagnostic antisera.
Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
Serological reactions - overview. Specificity and sensitivity. Titration of serum.
International units.
Direct and indirect agglutination.
Blood groups. Rheumatoid factor.
Precipitation. Double immune diffusion.
Radial immunodiffusion.
Turbidimetry, nephelometry.
Immunoelectrophoresis, immunofixation.
Blotting techniques.
Clinical significance of paraproteinemia.
Monoclonal antibodies.
Immunofluorescence tests.
Enzyme analysis (ELISA). RIA.
IgE determination.
Clinically significant organ specific and organ non-specific autoantibodies.
CD nomenclature of leukocyte antigens.
Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity.
Evaluation of phagocytic cells.
Immunophenotyping of leukemias.
Complement system tests.
Circulating and localised immune complexes.
HLA typing. Molecular genetic in immunology.
Skin tests for early hypersensitivity.
Delayed-type hypersensitivity tests. Anergy.
Prevention and treatment of allergic diseases.
Drug hypersensitivity.
Anaphylactic shock.
Indication and interpretation of immunological tests.

Literature

LITZMAN, Jiří, Martin PETŘEK and Vojtěch THON. Vyšetřovací metody v klinické imunologii (Investigation methods in clinical immunology). 1st ed. Brno: Masarykova universita, 1998, 45 pp. ISBN 80-210-1807-0. info

Assessment methods (in Czech)

Zápočet po absolvování povinné výuky

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.