VLIM051c Immunology - practice

Faculty of Medicine
Autumn 2014
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
prof. MUDr. Vojtěch Thon, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/02: Wed 10:10–11:50 ÚKIA N01021
VLIM051c/03: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/04: Wed 8:00–9:40 ÚKIA N01021
VLIM051c/05: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/06: Fri 10:10–11:50 ÚKIA N01021
VLIM051c/07: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/08: Fri 8:00–9:40 ÚKIA N01021
VLIM051c/09: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/10: Mon 10:10–11:50 ÚKIA N01021
VLIM051c/11: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/12: Mon 8:00–9:40 ÚKIA N01021
VLIM051c/13: Tue 10:10–11:50 ÚKIA N01021
VLIM051c/14: Tue 10:10–11:50 ÚKIA N01021, J. Litzman
VLIM051c/15: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/16: Thu 8:00–9:40 ÚKIA N01021
VLIM051c/17: Thu 10:10–11:50 ÚKIA N01021, V. Thon
VLIM051c/18: Thu 10:10–11:50 ÚKIA N01021
VLIM051c/30: No timetable has been entered into IS.
VLIM051c/31: No timetable has been entered into IS.
VLIM051c/32: No timetable has been entered into IS.
VLIM051c/33: No timetable has been entered into IS.
VLIM051c/34: No timetable has been entered into IS.
VLIM051c/35: No timetable has been entered into IS.
Prerequisites (in Czech)
VSBC0221p Biochemistry I - lect. && ( VSLC011p Med. Chemistry - lecture || VSLC021p Medical chemistry -l )&& VSBI0222p Biology II -lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. ) && VSFY0422s Physiology II - seminar && VSFY0422p Physiology II - lecture && ( VSBC041s Biochemistry II -s || VSBC041c Biochemistry II -pract. ) && ( VSLT0221s Bas. Med. Terminol. II -s || VSLT011s Bas. Med. Terminology -l ) && VSET021 Medical Ethics I && ZC041 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
  • LITZMAN, Jiří, Tomáš FREIBERGER, Vlastimil KRÁL and Vojtěch THON. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2. dotisk 1. vyd. Brno: Masarykova univerzita, 2011, 53 pp. ISBN 978-80-210-4227-8. info
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. 4th ed. Praha: Triton, 2009, 306 pp. ISBN 978-80-7387-280-9. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Presence in 90% of seminars with active participation in discussions is required. The knowledge is continuously checked by oral questions. The student must participate in ppreparation od crientific articles presentation or presentaion of case reports.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (Autumn 2014, recent)
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