aZLBC0321p Biochemisty I - lecture

Faculty of Medicine
autumn 2020
Extent and Intensity
2/0/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
RNDr. Hana Paulová, CSc. (lecturer)
Mgr. Jiří Slanina, Ph.D. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
MUDr. Michaela Králíková, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: Monika Šudáková
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Tue 10:10–11:50 A16/213
Prerequisites (in Czech)
aZLBF011c Med.Physics and Informatic-pr. && aZLBI0222c Biology II -practice && aZLLC011p Medical Chemistry,DM - lecture
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. The graduate is: acquainted with properties and function of enzymes, oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences. Understands the principles of cell bioenegetics. Becomes familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport. has knowledge on proteosynthesis including processes replication, transcription, translation and posttranslantion modifications. Understands the relation between the structure and function of proteins, is acquinted with function of hemoglobine in oxygen transport. Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.
Learning outcomes
At the end of the course the student will:
- understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
- describe the role of macro- and microbiogenic elements in the organism
- discusse the properties and function of enzymes
- describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism, and their relationships.
- understand the principles of energy production, utilization and deposition at the cellular level.
- explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
- describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understand the relationship between protein structure and function.
- explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
- discusse the principles of some diseases at the molecular level.
Syllabus
  • Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.
Literature
    required literature
  • FERRIER, Denise R. Biochemistry. Seventh edition. Philadelphia: Wolters Kluwer, 2017, viii, 567. ISBN 9781496363541. info
  • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Color atlas of biochemistry. 3rd ed., rev. and updated. Stuttgart: Thieme, 2013, ix, 495. ISBN 9783131003737. info
Teaching methods
Teaching form are lectures. Supplementary subjects are seminars.
Assessment methods
Student must have course-unit credit of seminar in the day of exam. Examination has writen and oral part. Writen part has 25 questions and is solved on computers in computer room of Department of Biochemistry. Limit for the oral part is 14/25. The second part is oral. Examination questions and other instructions you will find in the section Study materials of the course.

Changes in the organization of the exam in examination period Spring 2019/2020 valid since 1.6.2020:

The course of Biochemistry I (aZLBC0221p) is completed by an exam. The examination has only an oral part. The official list of examination questions published in the Study materials of aZLBC0221p is valid. Three questions will be randomly selected from the three parts of the list. There will be no preparation time before the oral exam. Students are supposed to summarize the substantial and relevant issues. Examiners may ask additional questions. If appropriate, students may use paper and a pen. Exams will take place in the seminar rooms of the Department of Biochemistry in the usual contact mode. During contact examinations, all currently issued hygienical and epidemiological precautions must be fully satisfied. Information will be given in the instructions to the exam that are part of exam dates in IS. Students that are abroad or cannot attend the exam from serious reasons may perform a distant examination using a video call in MS Teams platform, under the same conditions as in the contact exam. Students have the full responsibility for the proper choice and setup of the device for performing the distant exam (article 3a, paragraph 5, Decision of the Dean of Faculty of Medicine, No. 3/2020).
Language of instruction
English
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
The course is also listed under the following terms autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2020/aZLBC0321p