aZLBC0321p Biochemisty I - lecture

Faculty of Medicine
autumn 2023
Extent and Intensity
2/0/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
MUDr. Michaela Králíková, Ph.D. (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable
Wed 20. 9. to Wed 22. 11. Wed 10:00–11:50 B11/211, Wed 6. 12. to Wed 20. 12. Wed 10:00–11:50 B11/211; and Wed 29. 11. 10:00–11:50 C15/309
Prerequisites (in Czech)
aZLLC011p Med Chem, Dental Mat - lect && aZLBF011p Med.Physics and Informat.-lect && aZLBI0222p Biology II-lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. The graduate is: acquainted with properties and function of enzymes, oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences. Understands the principles of cell bioenegetics. Becomes familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport. has knowledge on proteosynthesis including processes replication, transcription, translation and posttranslantion modifications. Understands the relation between the structure and function of proteins, is acquinted with function of hemoglobine in oxygen transport. Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.
Learning outcomes
At the end of the course the student will:
  • understand the meaning of basic chemical concepts and can apply this knowledge to describe biochemical processes in the body and the properties of body fluids.
  • discusse the properties and function of enzymes.
  • describe basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism, and their relationships.
  • understand the principles of energy production, utilization and deposition at the cellular level.
  • explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
  • describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications.
  • understand the relationship between protein structure and function.
  • explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
  • discusse the principles of some diseases at the molecular level.
    • Syllabus
    • Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.
    Literature
      required literature
    • FERRIER, Denise R. Biochemistry. Seventh edition. Philadelphia: Wolters Kluwer, 2017, viii, 567. ISBN 9781496363541. info
      recommended literature
    • LIEBERMAN, Michael, Allan D. MARKS and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 4th ed. Baltimore: Lippincott, Williams & Wilkins, 2013, ix, 1014. ISBN 9781451100037. info
    • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Color atlas of biochemistry. 3rd ed., rev. and updated. Stuttgart: Thieme, 2013, ix, 495. ISBN 9783131003737. info
    • VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Textbook of biochemistry for dental students. 3rd edition. New Delhi: Jaypee, 2017, xiii, 289. ISBN 9789352701148. info
    Teaching methods
    Lectures.
    Assessment methods
    Students pass to continuing education in next semester without any duty of knowledge checking in this semester.
    Language of instruction
    English
    Further comments (probably available only in Czech)
    The course is taught annually.
    Information on the extent and intensity of the course: 30.
    Listed among pre-requisites of other courses
    The course is also listed under the following terms autumn 2020, autumn 2021, autumn 2022, autumn 2024.
    • Enrolment Statistics (autumn 2023, recent)
    • Permalink: https://is.muni.cz/course/med/autumn2023/aZLBC0321p