Bi7410 Protein Engineering

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Michal Vašina, Ph.D. (lecturer)
doc. Mgr. David Bednář, Ph.D. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Michal Vašina, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The aim of this course is to introduce methods and strategies commonly used in protein engineering to improve catalytic properties of enzymes.
Learning outcomes
At the end of the course, students will be able to:
- understand principles and explain differences between rational design, directed evolution, and semi-rational design;
- prepare the design of a protein engineering strategy with the aim to improve properties of studied protein;
- get a general knowledge about searches of novel genes/proteins in bioinformatics databases;
- explain the principles of methods of isolation, expression, and purification of proteins;
- explain principles and select a biophysical method suitable for analysis of secondary, tertiary and quaternary structure of proteins;
- describe the various screening techniques used for selection and/or screening of novel protein variants with improved properties
Syllabus
  • 1. Protein applications, targets of protein engineering, protein biosynthesis, protein structure and function and structure-function relationships.
  • 2. Identification of putative enzymes in sequence databases, bioinformatic analysis.
  • 3. Isolation of genes from host organisms, cloning, preparation of recombinant proteins, host organisms, protein expression and protein purification, protein sample quality control.
  • 4. Functional and structural characterization of proteins, protein interactions, enzyme catalysis, factors influencing the speed of enzymatic reaction, an overview of techniques for the analysis of protein secondary, tertiary structure and quaternary structure.
  • 5. Protein engineering approaches, advantages and limitations, overview of mutagenesis techniques.
  • 6. Directed evolution, screening and selection of mutants.
  • 7. In silico mutagenesis and semi-rational design.
  • 8. Rational design, prediction of the structure of enzyme variant, evaluation of the effect of mutations on enzyme structure and function.
  • 9. Examples of application of protein engineering: Directed evolution.
  • 10. Examples of application of protein engineering: Rational design.
Literature
    recommended literature
  • Protein engineering handbook. Edited by Stefan Lutz - Uwe Bornscheuer. Weinheim: Wiley-VCH, 2009, xli, 409-9. ISBN 9783527318506. info
    not specified
  • Directed evolution library creation : methods and protocols. Edited by Frances Hamilton Arnold - George Georgiou. Totowa, N.J.: Humana Press, 2003, x, 224. ISBN 1588292851. info
  • FERSHT, Alan. Structure and mechanism in protein science :a guide to enzyme catalysis and protein folding. New York: W.H. Freeman, 1998, xxi, 631 s. ISBN 0-7167-3268-8. info
Teaching methods
Lectures, class discussion.
Assessment methods
Final written test consists of 25 questions and is scored on a 25-point scale. A minimum score of 13 is required to successfully pass the exam.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024.
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