PřF:Bi1050 Biological techniques - Course Information

Bi1050 The Study of Animal and Plant Structure for Biology Teachers

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Jaroslava Dubová, CSc. (seminar tutor)
Ing. Martina Hájková (seminar tutor)
RNDr. Božena Koubková, Ph.D. (seminar tutor)

Guaranteed by

RNDr. Jaroslava Dubová, CSc.
Faculty of Science
Contact Person: Eliška Karasová

Timetable of Seminar Groups

Bi1050/01: Thu 10:00–11:50 Bpr,02004, Thu 10:00–11:50 Z3,02045
Bi1050/02: Mon 13:00–14:50 Bpr,02004, Mon 13:00–14:50 Z3,02045
Bi1050/03: Thu 8:00–9:50 Z3,02045, Thu 8:00–9:50 Bpr,02004
Bi1050/04: Thu 12:00–13:50 Z3,02045, Thu 12:00–13:50 Bpr,02004

Prerequisites (in Czech)

! Bi2302 Zoology microtechnique && ! Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Biology with a view to Education (programme PřF, B-BI)

Course objectives

Introduction to the Light Microscope Structure and Function, Techniques of Preparations. Botanical Techniques: Replicas. Cleared Preparations. Squash Preparations. Histology and Histochemistry. Phase Contrast. Documentation. Zoological Techniques: Native Preparations, Vital Staining. Whole Mounts, Sections, Squashes, Dry Mounts. Fixation. Staining. Mounting Media.

Syllabus

• Introduction. Light Microscope: Structure and Function. Köhler Illumination. Botanical Techniques: Replicas for Study by Light Microscope, Stoma Structure and Position, Subsidiary cells. Whole Mounts of Cleared Preparations, Development of Angiosperm Embryos. Squash Preparations: Cell Cycle, Mitosis, Chromosome Counting, Chromosome Doubling. Anatomical Methods: Sectioning (Hand Sections, Fixation, Infiltration and Embedding, Microtome Sections), Staining. Phase Contrast. Documentation. Zoological Techniques: Native Preparations. Vital Staining. Whole Mounts, Sections, Squashes, Dry Mounts. Fixation. Fixatives. Staining. Aqueous Media. Non-aqueous Mounting Media.

Literature

• KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
• PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
• LELLÁKOVÁ, Františka. Zoologická technika. 1. vyd. Praha: Universita Karlova, 1985, 122 s. info
• NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi2302 Zoological microtechnique .
  (!Bi1050) && (!NOW(Bi1050))  

• Enrolment Statistics (Autumn 2004, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2004/Bi1050