PřF:Bi1050 Biological techniques - Course Information
Bi1050 The Study of Animal and Plant Structure for Biology Teachers
Faculty of ScienceAutumn 2016
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- RNDr. Milan Baláž, Ph.D. (seminar tutor)
Mgr. Michal Benovics, Ph.D. (seminar tutor)
Mgr. Eva Řehulková, Ph.D. (seminar tutor) - Guaranteed by
- Mgr. Eva Řehulková, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Eva Řehulková, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi1050/01: Mon 19. 9. to Sun 18. 12. Thu 7:00–8:50 C13/332, Thu 7:00–8:50 D36/223, M. Baláž, E. Řehulková
Bi1050/02: Mon 19. 9. to Sun 18. 12. Thu 12:00–13:50 C13/332, Thu 12:00–13:50 D36/223, M. Baláž, E. Řehulková - Prerequisites (in Czech)
- ! Bi2302 Zoological microtechnique && ! Bi1301 Microscopic Techniques && (!NOW( Bi2302 Zoological microtechnique )) && (!NOW( Bi1301 Microscopic Techniques ))
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end of this practical course, students should be able
- to use various techniques of light microscopy efficiently
- to prepare various kinds of botanical or zoological mounts
- to take and efectivelly process microphotographs
- to utilize some simple techniques of image analysis
- to conduct simple biological experiment including the statistical treatment of the data
- to utilize basic facts about mitosis, embryogenesis and the other biological processes in their own teaching practice - Syllabus
- Introduction. Light microscope: structure and function. Köhler illumination. Botanical techniques: Microrelief technique for the study of the stomata structure and density. Whole mounts of cleared preparations: development of Angiosperm (dicotyledonous plant) embryo. Squash preparations: cell cycle, mitosis, chromosome counting. Anatomical methods: sectioning (hand sections, fixation, infiltration and embedding, microtome Sections), staining. Documentation - hand drawing, microphotographs and their processing. Simple technique of image analysis - leaf area measurement. Design and statistical treatment of simple biological experiments. Zoological techniques: Native preparations. Vital staining. Whole mounts, sections, squashes, dry mounts. Fixation. Fixatives. Staining. Aqueous media. Non-aqueous mounting media.
- Literature
- recommended literature
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- LELLÁKOVÁ, Františka. Zoologická technika. 1. vyd. Praha: Universita Karlova, 1985, 122 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- Teaching methods
- Practical training.
- Assessment methods
- Whole mounts must be prepared to pass the zoological part of the course. Botanical part of the course: elaboration of all protocols, written test, 75% of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Teacher's information
- http://www.sci.muni.cz/kfar/html/biologicka_technika_navody.htm
- Enrolment Statistics (Autumn 2016, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2016/Bi1050