PřF:CB070 Protein crystallography - Course Information
CB070 Protein crystallography
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
- Guaranteed by
- doc. RNDr. Jaromír Marek, Ph.D.
Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 12 fields of study the course is directly associated with, display
- Course objectives
- Symmetry of matter, point groups, symmetry of diffraction image, Laue classes, primitive and Bravais cell, space groups.
Diffraction of X-ray, Thompson scattering, interference of scattered waves, atomic and structure factor, Bragg and Laue equations, Ewald sphere, intensity of diffracted X-ray, Fourier synthesis
Diffraction experiment, sources of X-ray, synchrotrons, detectors, diffractometers, cryocrystallography.
Indexation, integral intensity, reduction, Lorenz, polarisation and absorption correction
Phase problem, Patterson function and method, probability distribution of structure factors, direct methods, R-factors, least-square-method, SHELXS a SHELXL.
Crystallisation of macromolecules, methods of sitting and hanging drop, seeding.
Phase problems and proteins, molecular replacements, methods of heavy atom derivatives, (SIR, MIR, MIRAS), MAD and selenoproteins selenoproteins.
Molecular replacement (MR).
Maps of electron density. Fourier transformations. Building of the model and model refinement. Least-square-method, constrains, restrains.
Protein. crystallography in post-genomic era
Crystallographic databases. - Syllabus
- Symmetry of matter. Interaction of X-ray with matter
- Diffraction by crystal
- Sources and detectors of X-ray. Diffractometer
- Phase problem
- Patterson and direct methods
- Refinement, R-factors, Least-square-method
- Programs SHELXS and SHELXL, CNS and CCP4.
- Crystallisation of protein crystals
- Methods of heavy derivatives of proteins
- Molecular replacement
- Refinement of protein structures
- Crystallographic databases.
- Literature
- MAREK, Jaromír and Z. TRÁVNÍČEK. Monokrystalová rentgenová strukturní analýza (Single crystal X-ray structure analysis). první. Olomouc: Vydavatelství Univerzity Palackého, 2002, 169 pp. nedělí se na edice. ISBN 80-244-0551-2. info
- GIACOVAZZO, C. Fundamentals of Crystallography. 1992. ISBN 0-19-855578-4. info
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2005, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2005/CB070