CB070 Protein crystallography

Faculty of Science
Autumn 2023
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jaromír Marek, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Tue 12:00–12:50 C04/211
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 12 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to explain to students the main principles of single crystal structural analysis, the theoretical background of diffraction on single crystal, the technical equipment used during diffraction experiments and the basic steps and methods used during processing of experimental data and solving of protein structures. The course CB070 is closely connected to exercise CB080.
Learning outcomes
At the end of this course, student will be: - able to understand and explain basic principles of monocrystal x-ray crystallography - introduced to history of x-ray crystallography - introduced to x-ray crystallography instrumentation - introduced to basic methodology of x-ray crystallography - introduced to practical examples from the fiel
Syllabus
  • Symmetry of matter. Interaction of X-ray with matter
  • Diffraction by crystal
  • Sources and detectors of X-ray. Diffractometer
  • Phase problem
  • Patterson and direct methods
  • Refinement, R-factors, Least-square-method
  • Programs SHELXS and SHELXL, CNS and CCP4.
  • Crystallisation of protein crystals
  • Methods of heavy derivatives of proteins
  • Molecular replacement
  • Refinement of protein structures
  • Crystallographic databases.
Literature
  • MAREK, Jaromír and Z. TRÁVNÍČEK. Monokrystalová rentgenová strukturní analýza (Single crystal X-ray structure analysis). první. Olomouc: Vydavatelství Univerzity Palackého, 2002, 169 pp. nedělí se na edice. ISBN 80-244-0551-2. info
  • GIACOVAZZO, C. Fundamentals of Crystallography. 1992. ISBN 0-19-855578-4. info
Teaching methods
Lectures. Home exercises at PC.
Assessment methods
Oral exam. Home exercises at PC.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2023/CB070