PřF:C7995 Adv. Biomolecular NMR - Course Information
C7995 Advanced Methods of Biomolecular NMR
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Radovan Fiala, CSc. (lecturer)
prof. Mgr. Lukáš Žídek, Ph.D. (lecturer) - Guaranteed by
- doc. RNDr. Radovan Fiala, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Radovan Fiala, CSc. - Prerequisites
- Students should have good knowledge of NMR theory at the level of the courses C5320 and/or C6770.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- The students will get hands-on knowledge of sofisticated experiments used in modern NMR spectroscopy with an accent on techniques for spectroscopy of proteins and nucleic acids. All important exprimental issues from sample preparation and spectrometer setup and calibration trough data aquisition and processing up to spectra evaluation will be discussed as well as practically performed in the laboratory.
- Syllabus
- 1. NMR sample, preparation and handling 2. Spectrometer setup 3. Spectrometer calibrations 4. Building blocks of pulse sequences 5. Programming of pulse sequences 6. Water suppression techniques 7. Homonuclear 2D experiments 8. Heteronuclear double and triple resonance experiments 9. 3D experiments for proteins and nucleic acids (HNCO, HNCa, HCCH-TOCSY, HCN) 10. Data processing 11. Analysis of protein NMR spectra 12. Analysis of NMR spectra of nucleic acids
- Literature
- NMR of macromolecules : a practical approach. Edited by Gordon C.K. Roberts. Oxford: Oxford University Press, 1993, 399 s. ISBN 0-19-963224-3. info
- Assessment methods (in Czech)
- Presence in practical sessions at the spectrometer is required. Final examination will be based on results of individual projects.
- Language of instruction
- English
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks. - Teacher's information
- http://ncbr.chemi.muni.cz/~fiala/AdvancedMethods.html
- Enrolment Statistics (Autumn 2007 - for the purpose of the accreditation, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2007-forthepurposeoftheaccreditation/C7995