C7995 Advanced Methods of Biomolecular NMR

Faculty of Science
Autumn 2007
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Radovan Fiala, CSc. (lecturer)
prof. Mgr. Lukáš Žídek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Radovan Fiala, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Radovan Fiala, CSc.
Prerequisites
Students should have good knowledge of NMR theory at the level of the courses C5320 and/or C6770.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The students will get hands-on knowledge of sofisticated experiments used in modern NMR spectroscopy with an accent on techniques for spectroscopy of proteins and nucleic acids. All important exprimental issues from sample preparation and spectrometer setup and calibration trough data aquisition and processing up to spectra evaluation will be discussed as well as practically performed in the laboratory.

Syllabus
  • 1. NMR sample, preparation and handling

    2. Spectrometer setup 3. Spectrometer calibrations 4. Building blocks of pulse sequences 5. Programming of pulse sequences 6. Water suppression techniques 7. Homonuclear 2D experiments 8. Heteronuclear double and triple resonance experiments 9. 3D experiments for proteins and nucleic acids (HNCO, HNCa, HCCH-TOCSY, HCN) 10. Data processing 11. Analysis of protein NMR spectra 12. Analysis of NMR spectra of nucleic acids
Literature
  • NMR of macromolecules : a practical approach. Edited by Gordon C.K. Roberts. Oxford: Oxford University Press, 1993, 399 s. ISBN 0-19-963224-3. info
Assessment methods (in Czech)
Presence in practical sessions at the spectrometer is required. Final examination will be based on results of individual projects.
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
Teacher's information
http://ncbr.chemi.muni.cz/~fiala/AdvancedMethods.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Autumn 2018, Spring 2019, Autumn 2019, Spring 2020, Autumn 2020, Spring 2021, autumn 2021, Spring 2022, Autumn 2022, Spring 2023, Autumn 2023, Spring 2024, Autumn 2024, Spring 2025.
  • Enrolment Statistics (Autumn 2007, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2007/C7995