C7995 Practical NMR Spectroscopy of Biomolecules

Faculty of Science
Spring 2025
Extent and Intensity
1/0/1. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching
Teacher(s)
doc. RNDr. Radovan Fiala, CSc. (lecturer)
doc. Mgr. Karel Kubíček, PhD. (lecturer)
Mgr. Pavel Kadeřávek, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Radovan Fiala, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Radovan Fiala, CSc.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
Students should have basic knowledge of NMR theory, previous passing the course C5320 is an advantage but not absolutely necessary.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The students will get hands-on experience with using NMR spectrometers with an accent on techniques for spectroscopy of proteins and nucleic acids. All important experimental issues from sample preparation and spectrometer setup and calibration trough data acquisition and processing up to spectra evaluation will be discussed as well as practically performed in the laboratory. At the end of the course, the students will be able to use independently the sophisticated NMR instrumentation for routine measurements of spectra of proteins and nucleic acids, as well as most other common samples.
Learning outcomes
At the end of the course, the students will be able to use independently the sophisticated NMR instrumentation for routine measurements of spectra of proteins and nucleic acids, as well as most other common samples.
Syllabus
  • 1. NMR sample, preparation and handling 2. Spectrometer setup 3. Spectrometer calibrations 4. 1D proton spectroscopy and water suppression techniques 5. 1D NMR spectroscopy of nuclei other than H-1 6. Homonuclear 2D experiments 7. Heteronuclear experiments for natural abundance and isotopically labeled samples 8. Data processing, apodization functions, linear prediction, non-uniform sampling 9. Analysis of protein NMR spectra 10. Analysis of NMR spectra of nucleic acids
Literature
    recommended literature
  • CAVANAGH, John. Protein NMR spectroscopy : principles and practice. 2nd ed. Amsterdam: Elsevier, 2007, xxv, 885. ISBN 9780121644918. info
  • BERGER, Stefan and Siegmar BRAUN. 200 and more NMR experiments : a practical course. Weinheim: Wiley-VCH, 2004, xv, 838. ISBN 3527310673. info
  • NMR of macromolecules : a practical approach. Edited by Gordon C.K. Roberts. Oxford: Oxford University Press, 1993, 399 s. ISBN 0-19-963224-3. info
Teaching methods
Lectures plus laboratory practice
Assessment methods
Written test of NMR theory plus test of practical operation of the NMR spectrometer.
Language of instruction
English
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
Teacher's information
http://www.ncbr.muni.cz/~fiala/
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Autumn 2018, Spring 2019, Autumn 2019, Spring 2020, Autumn 2020, Spring 2021, autumn 2021, Spring 2022, Autumn 2022, Spring 2023, Autumn 2023, Spring 2024, Autumn 2024.
  • Enrolment Statistics (recent)
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