PřF:Bi5599 App.Chem.and Biochem. - Course Information

Bi5599 Applied Chemistry and Biochemistry

Extent and Intensity

2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer), MVDr. Mgr. Monika Dušková, Ph.D. (deputy)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.

Timetable

Thu 9:00–10:50 BFU

Prerequisites (in Czech)

Bi4020 Molecular biology && C3580 Biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand the importance of individual chemical/biochemical techniques and to interpret both their own experimental data, based on the techniques taught in the course. They should be able to work with the information in the primary literature, in order to understand and implement the techniques described in primary scientific literature in the area of animal physiology and molecular physiology. They should make reasoned decisions about the importance of the data and to interpret their biological importance. Based on this, they will be able to present and explain the techniques used in their own work to expert audience.

Syllabus

• 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
• 2)Basic immunochemistry techniques.
• 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
• 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
• 5)Lipids and polysacharidy; isolation, separation and identification.
• 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
• 7)Specific cellular assays.
• 8)Fluorescennce techniques.
• 9)Aplication of in vivo mammalian models in physiology.

Literature

• Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
• Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
• Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000

Teaching methods

lectures, class discussion

Assessment methods

Final seminar. Each student is required to prepare a presentation about methodical approaches used in an assigned paper.

Language of instruction

Czech

Further Comments

Study Materials

Listed among pre-requisites of other courses

• Bi5599c Applied Biochemistry and Cell Biology Methods - practice
  Bi3030 && NOW(Bi5599)  

• Enrolment Statistics (Autumn 2010, recent)
  
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