Bi5599 Applied Chemistry and Biochemistry

Faculty of Science
Autumn 2015
Extent and Intensity
2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 9:00–10:50 B11/235
Prerequisites (in Czech)
Bi4020 Molecular biology && C3580 Biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand the importance of individual chemical/biochemical techniques and to interpret both their own experimental data, based on the techniques taught in the course. They should be able to work with the information in the primary literature, in order to understand and implement the techniques described in primary scientific literature in the area of animal physiology and molecular physiology. They should make reasoned decisions about the importance of the data and to interpret their biological importance. Based on this, they will be able to present and explain the techniques used in their own work to expert audience.
Syllabus
  • 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
  • 2)Basic immunochemistry techniques.
  • 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
  • 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
  • 5)Lipids and polysaccharides; isolation, separation and identification.
  • 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
  • 7)Specific cellular assays.
  • 8)Fluorescence techniques.
  • 9)Application of in vivo mammalian models in physiology.
Literature
  • Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
  • Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
  • Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000
Teaching methods
lectures, class discussion
Assessment methods
The course is finished as colloquium. Each student is required to prepare a short presentation (10 minutes) about methodical approaches used in an assigned scientific paper. Students summarize in their presentation both foundations and results of the assigned paper, and tehy commentin deatil the methodology used - a specific method is described by them according to the selection made by lecturers. Final evaluation is based on the quality of presentation and on the outcome of discussion with lecturers.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2015, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2015/Bi5599