PřF:Bi7030c Bacterial physiology - pr. - Course Information
Bi7030c Bacterial physiology - practical course
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 0/5/0. 5 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Mgr. Andrea Vávrová, Ph.D. (seminar tutor) - Guaranteed by
- doc. RNDr. Miroslav Němec, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi7030c/01: Wed 7:00–11:50 Bpb,02012, M. Krsek, A. Vávrová
Bi7030c/02: Wed 15:00–19:50 Bpb,02012, M. Krsek, A. Vávrová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to understand principals of work with bacterial cells and bacteriophages and to understand chosen tasks, to solve various problems they can face in bacterial physiology; to use set of chosen techniques used in physiological laboratories; to work with growth characteristics of bacterial cells and phages, influence of temperature and UV on lysogenic cells and phages; to prepare crude enzymatic preparation and to test its enzymatic activity.
- Syllabus
- Determination of the bacterial proteins and dry-weight. Bacterial growth curve in static culture (using plate counts). One-step growth curve of the polyvalent and virulent staphylopage. Determination of dehydrogenase activity. Preparation of the crude enzymatic preparation (HEP) with phosphatase activity. Determination of phosphatase activity of native cells and HEP. Oxygen consumption (Oxi-Top). Biodegradation. Influence of heavy metals on bacterial growth (Bioscreen).
- Literature
- Schemata pro jednotlivé úlohy poskytne vyučující
- Horáková, D., Němec, M. (2003) : Laboratorní cvičení z fyziologie bakterií
- Teaching methods
- Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
- Assessment methods
- Active participation at practical course, reports, credit.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2012, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2012/Bi7030c